白术糖复合物提取纯化工艺研究

Extraction and Purification of Atractylodes macrocephala Monosaccharide Composition

目的：研究白术糖复合物提取纯化方法。方法：水提法提取白术糖复合物，中性氧化铝、树脂吸附法进行精制纯化，考察不同方法的除杂效果，确定工艺参数；盐酸紫外分光光度法、蒸发光散射法检测提取物中的糖含量。结果：白术提取液经中性氧化铝精制后糖复合物得率与纯度分别为23％～25．5％和56％～60％；根据D900弱碱性阴离子交换树脂、D201阴离子交换树脂、AB-8大孔吸附树脂的静态脱色曲线，它们对白术提取液色素杂质的交换能力大小为D900〉AB-8〉D201；D900离子交换树脂吸附法精制白术糖复合物得率为17％以上，盐酸紫外分光光度法检测糖纯度在76％以上。结论：水提后D900离子交换树脂吸附法精制白术糖复合物效果较好，糖定量分析以盐酸紫外分光光度法较为理想。

Objective ： To study the method of Atractylodes macrocephala monosaccharide composition （AMMC） ＇s extraction, purification and analysis. Methods： To extract AMMC with water extraction method and purify it with neutro-alumina and resin adsorption method, to observe removal impurity effect of several methods and determinethe technology parameters. To detect monosaccharide＇s content in AMMC with hydrochloric acid-ultraviolet spectrophotometric determination and Evaporative light scattering detector （ELSD）. Results： After purificated with neutro-alumina, the yield and purity of AMMC were 23% -25.5% and 56% -60%, respectively. According to the static decoloring curve of D900,D201 and AB-8 resins, their decolorization effect＇s rank was D900 〉 AB-8 〉 D201. The yield of AMMC purificated with D900 ion exchange resin was above 17% , and the purity of monosaccharide was above 76%. Conclusion： The effect of AMMC purifieated with D900 ion exchange resin after water extration is much better, the ideal method for the quantitative analysis of monosaccharide is hydrochloric acid-ultraviolet spectrophotometric determination.