摘要
目的探讨肿瘤坏死因子TNFα-238G/A基因多态性与变应性鼻炎的关系。方法用酶免法测定TNF水平,运用多聚酶链反应技术检测52例变应性鼻炎及89例正常人肿瘤坏死因子TNFα-238G/A基因多态性。结果变应性鼻炎组TNFα-238G/A、G/G、A/A表型频率分别为:0.7885、0.2115、0,对照组分别为0.9551、0.0449、0;变应性鼻炎组TNFα-238G、TNFα-238A基因频率分别为0.8679、0.1321。对照组分别为0.8679、0.0225,肿瘤坏死因子TNFα-238G/A各种基因型频率在患者组与正常对照组之间的差异有显著性(p<0.05),而血浆中TNF水平,变应性鼻炎组明显高于对照组,两组之间比较差异有显著性意义(p<0.05)。结论血清TNF水平显著升高,提示炎性反应在变应性鼻炎病程中有重要作用,肿瘤坏死因子TNFα-238G/A基因多态性与变应性鼻炎有明显相关。
Objective To investigate the relationship between serum levels of tumor necrosis factor（TNF） or its polymorphism and allergic rhinitis. Methods Serum level of TNF was measured by R/A, and the polymorphism of TNFα - 238G/A gene was screened by PCR - RELP. Results The gene frequency of TNFα - 238G/A in AR group was as followed. TNFα - 238G/A,G/G,A/A genotype frequency ：0. 7885,0.2115,0. TNFα - 238 G, TNFα - 238 A Gene frequency：0. 8679,0. 1321. The gene frequency of TNFα -238G/A in control group was as followed. TNFα - 238G/G,G/A,A/A genotype frequency：0. 9551,0. 0449,0. TNFa-238 G,TNFa -238 A Gene frequency：0. 9775, 0. 0225 ,There were significant differences in the frequencies of TNFα - 238G/A gene between the patients and control group（p 〈0. 05） ,allergic rhinitis group showed significantly higher serum level of TNF than control group（p 〈 0. 05 ）. Conclusion Serum TNF increased in allergic rhinitis, it suggest that TNF play important role in the process of allergic rhinitis and evident association was found between TNFα -238G/A polymorphism and allergic rhinitis.
出处
《现代医院》
2009年第5期22-24,共3页
Modern Hospitals
