摘要
对圆弧青霉ＰＧ３７发酵产酶工艺及脂肪酶提取工艺等进行了系统的研究。实验结果表明，２０ｍ３发酵罐在通风量为０．３～０．８Ｖ／Ｖ／ｍｉｎ、搅拌转速为１８０ｒ／ｍｉｎ、发酵温度为２８℃、培养过程中流加棉籽油以控制发酵醪ｐＨ值为６．５～７．０的条件下，发酵８４ｈ左右，成熟发酵醪酶活为４５２０Ｕ／ｍＬ。发酵液经过滤、超滤浓缩、硫铵沉淀、造粒等过程制得颗粒碱性脂肪酶成品，酶的提取总收率在７０％以上，颗粒酶活力单位为５．０×１０４Ｕ／ｇ。
The enzymeproducing fermentation process and lipase recovery with penicillium cyclopium PG37 were studied systematically.The results showed that with the conditions of the air flow rate 0.3～0.8V/V/min,agitation speed 180 r/min,fermentation temperature 28 ℃ and fermentation time about 84 hours,the lipase yield reached 4 520 U/mL,during which the cotton seed oil was fed to control pH value of culture broth at 6.5～7.0.The granulated alkaline lipase product was prepared by filtration,ultrafiltration,ammonium sulfate precipitation and granulation.The overall recovery of lipase and the granulated enzyme activity were over 70% and 5×10~4 U/g respectively.
基金
国家"八五"科技攻关项目
关键词
碱性脂肪酶
圆弧青霉PG37
发酵
过滤
脂肪酶
alkaline lipase
penicillium cyclopium PG37
ultrafiltration
granulated enzyme
