摘要
利用硫酸铵分级盐析、SephadexG-75凝胶柱层析分离纯化了云芝菌丝体中的超氧化物歧化酶(SOD),在2个吸收峰处收集到具有酶活的SOD,纯化了12.6倍,总回收率为50.6%,获得SOD酶比活为4.61U/mg.
The SOD of Coriolus versicolor mycelium was purified by salting out with ammonium sulfate and SephadexG-75 gel column chromatography. The SOD collected at two absorption bands was purified by 12.6 times, with 50.6% recovery of enzyme activity and 4.61U/mg of specific activity.
出处
《深圳职业技术学院学报》
CAS
2009年第3期50-52,共3页
Journal of Shenzhen Polytechnic
基金
深圳职业技术学院重点科技开发项目(01KJb016)
关键词
云芝菌丝体
SOD
纯化
Coriolus versicolor mycelium
SOD
purification
