肝脏细胞在体外培养过程中消长变化的探索

EXPLORING THE HEPATOCYTER′S SWING OF THE PENDULUM′S CHANGE IN VITRO

为获得长期存活的肝细胞，采用肝细胞组织块培养法和肝细胞直接分离培养法分别培养雄性幼龄、壮龄、老龄小鼠肝细胞，结果证明这两种方法均能够成功地培养出原代肝细胞，通过比较发现对雄性壮龄小鼠肝细胞直接分离培养法较适合于体外原代培养，实验得到最佳的培养基础体系为：培养温度37．0℃，胰蛋白酶用量20．0mL，消化时间15．0min，雄性壮龄小鼠（20．0～25．0g）．

In order to obtain long-term survival of hepatocytes, this paper we use the method of planting culture of hepatic tissues and pancreatin digestion of hepatic tissues to culture the male mouse of the young, the prime of life and the old. The ues of these two methods for mouse hepatocyes culture proved to be successful and primary culture of new-born mouse hepatocytes has been established. By discussed the differences between two methods we find that the male mouse of the prime of life＇s culture by pancreatin digestion of hepatic tissues adapts to the primary culture in vitro. The results showed that culture temperature 371 0 ℃, amount of tryps in 20. 0 mL, digestion time 15. 0 min, male mouse of the prime Of life＇s （20.0-25.0 g）,pancreatin digestion of hepatic tissues.