大鼠角膜移植后新生血管演变观察(英文)

Neovascularization profile in rat allogenic penetrating keratoplasty

目的:观察大鼠同种异体穿透性角膜移植后无干预下角膜新生血管的生长演变。方法:SPF级雌性SD大鼠34眼行同种异体穿透性角膜移植,术后4,7,15和30d在显微镜下观察角膜新生血管的生长情况,通过公式C/12×3.14×[r2-(r-I)2]计算角膜新生血管的面积。结果:在移植的34眼中出现新生血管的29眼(85%)。新生血管最初环角膜缘呈毛刷状生长,后向角膜中央蔓延,为扭曲粗大的血管,末端呈树枝状分支。高峰时期交叉密布成网状,以后逐渐萎缩。角膜移植后4,7,15和30d角膜新生血管平均面积分别为11.8±3.5mm2,18.5±4.0mm2,14.4±4.3mm2和6.0±1.8mm2,总体平均面积为12.7±1.9mm2;新生血管面积占角膜面积百分比值分别为30.8%±8.7%,65.3%±12.8%, 59.4%±14.5%和36.2%±10.9%,总体为48.7%±6.4%。结论:大鼠同种异体穿透性角膜移植后角膜新生血管在第4d时出现,7d时面积达最大值;以后逐渐下降,30d时约降至7d时面积的1/2。

AIM： To observe the neovascularization process with no intervention in rat allogenic penetrating keratoplasty. METHODS. AIIogenic penetrating keratoplasties were successfully performed in 34 female SPF SD rats with no intervention after operations. Corneal neovascularization （CNV） process was noted on day 4, 7, 15 and 30 with operating microscope. The vascular area surface was calculated using the formula C/12 × 3.14 ×[ r^2-（r-I）2].RESULTS. CNV was noted in 29 out of 34 rats （85%）. Firstly, the new vessels distributed around the cornea like a brush then gradually extended towards the center. The vessels were distorted and massive with branched tails, they continued growing to reticulated veins in peak time then gradually atrophied. The average neovascularization area （SE） on day4, 7, 15 and 30 was 11.8±3.5mm^2, 18. 5 ± 4. 0mm^2, 14. 4 ± 4. 3mm^2 and 6. 0 ± 1. 8mm^2 respectively and 12. 7 ± 1. 9mm^2 in total. The average percentage that new vessels accounting the whole cornea area（SE） was 30. 8% ±8.7%, 65.3% ±12.8%, 59. 4% ± 14.5%,36.2% ±10.9% and 48.7% ±6.4% in total. CONCLUSION. In rat allogenic penetrating keratoplasties without intervention, CNV presented on day 4 and reached the maximum area on day 7. Then the vessels gradually atrophied, about 50% of the maximum area still remained on day 30.