糖尿病大鼠白内障免疫球蛋白的实验研究

Experimental study of immunoglobulin in rat diabetic cataract

目的:糖尿病白内障大鼠不同时间点房水中免疫球蛋白变化,探讨糖尿病性白内障形成的作用机制。方法:以链脲佐菌素建成大鼠糖尿病模型,观察不同时期晶状体的变化情况,利用全自动生化检测仪检测各时间点大鼠房水中免疫球蛋白。结果:糖尿病大鼠晶状体逐渐混浊并随时间推移而加重。正常对照组大鼠房水中IgG含量为0.29±0.01mg/mL,成模后1,2,3mo大鼠房水中IgG含量分别为0.74±0.02,0.87±0.02及1.00±0.05mg/mL;正常对照组大鼠房水中IgA含量为1.67±0.01mg/mL,成模后1,2,3mo含量分别为4.23±0.01,4.42±0.01及10.56±0.07mg/mL;正常对照组大鼠房水中Alb含量为10.93±0.01mg/mL,成模后1,2,3mo含量分别为210.00±1.00,2200.00±2.64及5900.00±3.92mg/mL;正常对照组大鼠房水中C3含量为0,成模后1,2,3mo含量分别为10.00±0.01,10.00±0.01及50.00±0.06mg/mL。各组之间差异均具有统计学意义。结论:血-房水屏障发生改变,影响晶状体的正常代谢,是糖尿病性白内障形成的一个重要作用机制。

AIM. To investigate the mechanism of diabetic cataract. METHODS： The animal model of diabetes was introduced by streptozotocin. The changes of lens in different period were observed. Immunoglobulin in aqueous humor was tested using full automatic biochemistry detector. RESULTS： IgG, IgA, AIb and C3 of aqueous humor in diabetic rat was increased with time-lapse. In control group, IgG amount was 0.29 ±0.01mg/mL;The amount of IgG in diabetic rat was 0.74 ±0.02,0.87 ±0.02 and 1.00 ± 0.05mg/mL after 1, 2, and 3 months respectively. In control group, IgA amount was 1.67±0.01mg/mL; The amount of IgA in diabetic rat was 4. 23 ± 0. 01,4. 42 ± 0.01 and 10. 56 ± 0.07mg/mL after 1, 2, and 3 months respectively. In control group, Alb amount was 10.93 ± 0.01mg/mL,The amount of IgA in diabetic rat was 210.00 ± 1.00,2200.00 ± 2.64 and 5900.00 ± 3.92mg/mL after 1, 2, and 3 months respectively. In control group, C3 amount was 0. The amount of IgA in diabetic rat was 10.00 ±0.01,10.00 ±0.01 and 50.00 ±0.06mg/mL after 1,2, and 3 months respectively. There were significant differences among groups. CONCLUSION： Changing of blood-aqueous barrier affect the normal metabolism of the lens, this is an important mechanism of diabetic cataract formation.