作物AFLP差异片段的回收与克隆

Reclaiming and Cloning of Segments of AFLP in Peanut

通过高低油酸花生的种植、DNA的提取、双酶切、连接、预扩增、再扩增、制胶及差异片段的回收、纯化、克隆等一系列操作,摸索出一套AFLP分子标记下的分子克隆技术,适合于花生,也适合于其他经济作物。

Through of the following process： drawing DNA, enzyme-cuting, linking up, advance expand increasing, making film , reclaiming, cloning and so on. Finding the way of molecule clone at AFLP basis. The way is suitable not only in peanut and in other industrial crops.