超临界CO_2流体提取苦参中总黄酮

Extraction of Total Flavonoids in Sophora flavescens by Supercritical CO_2 Fluid

目的研究超临界流体法提取苦参中黄酮类化合物的最佳工艺。方法采用单因子及正交试验,考察提取温度、压力、时间以及夹带剂流量对苦参黄酮类化合物提取量的影响。结果苦参黄酮类化合物提取工艺的最佳参数组合为:温度30℃,压力30MPa,乙醇流速0.4mL/min,时间60min。最佳条件下总黄酮提取率5.82%。结论超临界CO2提取苦参中总黄酮工艺简单、提取率高,可替代已有的方法。

Objective To study the optimal extraction condition of total flavonoids in Sophoraflavescens by supercritical CO2 fluid. Methods The single-factor and orthogonal design experiments were adopted to investigate the effects of extracting temperature, pressure, time and the ethanol flow rate on the extraction efficiency. Results The optimal extraction conditions were as follows： extraction temperature of 30℃ , extraction pressure of 30 MPa, ethanol flow rate of 0.4 mL/min, extraction time of 60 rain. The extraction efficiency of total flavonoids was as high as 5.82 %. Conclusion With simple technology and high extraction efficiency, the supercritical CO2 fluid extraction for total flavonoids in Sophoraflavescens is feasible.