谷精草有效成分分析及体外抗菌活性测定

Studies on Effective Composition Analysis and in Vitro Antibacterial Activity of Eriocaulon Buergerianum Koern

采用试管法和圆形滤纸层析法分析鉴定了谷精草的化学成分,结果表明:谷精草含有生物碱、酚性成分、有机酸、黄酮及其甙类、挥发油、植物甾醇、鞣质等化学成分。用纸片法和试管法对谷精草水提取液进行了体外抗菌作用试验,结果表明:谷精草水提取液对金色葡萄球菌、链球菌、巴氏杆菌、沙门氏菌、大肠杆菌等兽医临床常见病原微生物都有较强的抗菌作用,抑菌圈直径分别为12.16±0.33mm,15.36±0.29mm,13.57±0.61mm,8.63±0.26mm,9.88±0.72mm;谷精草水提取液对金色葡萄球菌、链球菌、巴氏杆菌、沙门氏菌、大肠杆菌的最低抑菌浓度(MIC)分别为0.125g/ml、0.063g/ml、0.125g/ml、0.5g/ml、0.25g/m;最低杀菌浓度(MBC)分别为0.25g/ml、0.125g/ml、0.25g/ml、1g/ml、1g/ml。

The test tube method and isolated method of the circular filter paper chromatographic were used to identify the chemical compotion of Eriocaulon buergerianum koern.The results showed that it constains alkaloid, phenol, organic acid, flavonoids, glycoside, volatile oil, plant sterol, tannin and so on.The antiblastic experiment in vitro of Eriocaulon buergerianum Koern water extracts was investigated by tube dilution method and filter-paper method. The effect demonstracted： Eriocaulon buergerianum Koern water extracts have stronger bacteriostatic to Staphylococcus aureus, streptoc, Pasteurella, Salmonella, E.coli, the diameter of bacteriostatic ring to various pathogenic bacteria is 12.16 ± 0.33 mm, 15.36± 0.29 mm, 13.57±0.61 mm, 8.63±0.26 mm, 9.88±0.72 mm; MIC is 0.125 g/ml, 0.063 g/ml, 0.125 g/ml, 0.5 g/ml, 0.25 g/ml; and MBC is0.25 g/ml, 0.125g/ml, 0.25 g/ml. 1 g/ml, 1g,/m 1g/ml respectively.