绿色荧光蛋白基因在水稻细胞中的高效表达

EFFICIENT EXPRESSION OF GREEN FLUORESCENT PROTEIN GENE IN RICE CELLS

绿色荧光蛋白基因在水稻细胞中的高效表达＠许新萍＠黄粤＠卫剑文＠李宝健￥中山大学生物工程研究中心绿色荧光蛋白，水稻，瞬间表达，β－葡糖苷酸酶绿色荧光蛋白基因在水稻细胞中的高效表达许新萍黄粤卫剑文李宝健（中山大学生物工程研究中心广州５１０２７５）关键词绿色荧...

An expression vector suitable for rice (Oryza sativa L.), pAct1.GFP, was constructed by placing the improved green fluorescent protein (GFP) coding sequence under the control of the actin 1 (Act1) promoter of rice. Embryogenic rice calli derived from the mature embryos were bombarded with gold particles coated with pAct1.GFP. Expression of GFP gene in rice cells was followed by incidentlight fluorescence microscopy in vivo and in real time. Bright green fluorescence signals were visible under blue light 3 to 4 h following bombardment. After 10 to 16 h, the maximum expression of GFP gene was observed in 85% of the calli. Meanwhile, the expression of βglucuronidase (GUS) gene driven by Act1 promoter reached the highest in rice cells 40 to 48 h after transformation. GFP as a sensitive and vital reporter may be used as a replacement for GUS in rice transient expression system. However, no green fluorescence from stable expression of GFP gene was observed in transformants.