普通小麦细胞质雄性不育系及其保持系线粒体DNA的RAPD分析

USING RAPD TO ANALYSE mtDNA OF CMS LINE AND ITS MAINTAINER LINE IN COMMON WHEAT

应用随机扩增多态ＤＮＡ（ＲａｎｄｏｍａｍｐｌｉｆｉｅｄｐｏｌｙｍｏｒｐｈｉｃＤＮＡｓ，ＲＡＰＤ）技术，对普通小麦细胞质雄性不育系（Ａ）及其保持系（Ｂ）线粒体基因组（ｍｉｔｏｃｈｏｎｄｒｉａｌｇｅｎｏｍｅ）的指纹图谱进行了分析。共使用引物４１个，其中２０个引物得到了扩增产物，部分引物扩增结果表现多态性。说明线粒体ＤＮＡ（ｍｉｔｏｃｈｏｎｄｒｉａｌＤＮＡ，ｍｔＤＮＡ）序列在不育系和保持系之间存在差异，进一步明确了小麦细胞质雄性不育（ｃｙｔｏｐｌａｓｍｉｃｍａｌｅｓｔｅｒｉｌｅ，ＣＭＳ）与ｍｔＤＮＡ的关系。

Mitochondrial DNA (mt DNA) coming from cytoplasmic male sterile (CMS) line and its maintainer line of common wheat was studied by means of random amplified polymorphic DNAs (RAPD).The “fingerprints” of the amplification by polymerase chain reaction (PCR) of random DNA segments was analysed.Using 4 wheat variaties in conjunction with 41 primers show polymorphic.The results indicate the difference between CMS line and maintainer line and verified the relation between CMS and mtDNA.