黄芪对顺铂耳毒性保护作用的实验研究

The Protective Role of Radix Astragali against Ototoxicity Caused by Cisplatin

目的探讨黄芪是否对顺铂的耳毒性具有保护作用。方法健康SD大鼠40只随机分成4组,每组10只。对照组:生理盐水2ml/d腹腔注射6天;黄芪组:黄芪注射液5g·kg-1·d-1腹腔注射6天;顺铂组:顺铂4mg·kg-1·d-1腹腔注射6天;顺铂加黄芪组:腹腔注射黄芪5g·kg-1.d-1加顺铂4mg·kg-1·d-16天。用药前及用药后第7天行畸变产物耳声发射(DPOAE)检测,然后处死大鼠。每组半数动物冰冻连续切片,DNA末端转移酶介导的缺口末端标记法(TUNEL)检测毛细胞凋亡;半数动物扫描电镜观察毛细胞形态。结果顺铂组用药后DPOAE幅值下降,毛细胞受损,并可观察到凋亡细胞,与对照组及黄芪组比较差异具有显著统计学意义(P<0.01)。顺铂加黄芪组用药后DPOAE幅值上升,毛细胞受损减轻,凋亡细胞数量减少,与顺铂组比较,差异有统计学意义(P<0.05)。结论黄芪能有效保护耳蜗免受顺铂的耳毒性损伤。

] Objective To study the protective role against cisplatin--induced ototoxicity by using radix astra- gali in rats. Methods Forty rats were randomly divided into four groups. Group A received saline as controls. Group B received radix astragali. Group C received injection of cisplatin（4 mg/kg） as experiments for 6 days. Group D received both radix astragali（5 g/kg） and cisplatin（4 mg/kg）. Distortion product acoustic emission （DPOAE） was applied to each rat before and 7 days after cisplatin injection. All the animals were sacrificed on the 7th day, Half of the cochleas were observed by frozen section and the apoptosis of hair cells was detected by TUNEL meth- od. Scanning electron microscopy was utilized to evaluate the cochlear morphology of the other rats. Results The DPOAE amplitudes of Group C decreased significantly compared to the group A and group B（P〈0.01）. The dam- age and apoptosis of hair cells were noted in the group C and group D, while the hair cells of group A and group B showed no sign of apoptosis or damage, Compare to experimental group , the DPOAE amplitudes of group D were higher, and the damage and apoptosis of hair cells were significantly lower（P〈0.05）. Conclusion This study sug- gests that radix astragali can effectively reduce eisplatin ototoxicity.