阿魏酸钠对兔急性心肌梗死再灌注后无复流的影响

Effect of sodium ferulate on no-reflow in a rabbit model of acute myocardial infarction and reperfusion

目的探讨阿魏酸钠(sodium ferulate,SF)对兔急性心肌梗死(acute myocardial infarction,AMI)再灌注后无复流的影响。方法随机将24只成熟雄性新西兰大白兔分为假手术组、对照组、SF组3组,每组8只,分别建立AMI缺血再灌注模型,于缺血前5min、结扎后4h,再灌注后2h取静脉血。应用放射免疫法测定血清和心肌组织内皮素-1(endothelin-1,ET-1)浓度。采用酶联免疫吸附法(ELISA)测定血清白细胞介素-6(interleukin-6,IL-6)。应用硫黄素染色评估无复流面积。结果①对照组和SF组AMI后4h、再灌注后2h血清ET-1浓度高于假手术组,差异有统计学意义(P均<0.01);与AMI前比较,对照组及SF组AMI后4h、再灌注后2h的血清ET-1浓度均显著升高(P均<0.01);SF组AMI后4h、再灌注后2h的血清ET-1浓度均显著低于对照组(P均<0.01)。②对照组和SF组复流区和无复流区心肌组织中ET-1浓度均显著高于假手术组心肌组织(P均<0.01)。SF组复流区心肌组织中的ET-1浓度显著低于对照组(P均<0.01)。③对照组和SF组AMI后4h、再灌注后2h血清IL-6浓度显著高于假手术组(P均<0.01);与AMI前比较,对照组及SF组AMI后4h、再灌注后2h的血清IL-6浓度均显著升高(P均<0.01);SF组血清IL-6浓度显著低于对照组(P均<0.05)。④病理染色分析示,SF组无复流区心肌范围为57.54%±8.36%,与对照组83.91%±5.35%相比显著降低(P<0.01)。结论SF能有效保护兔内皮功能,抑制炎症反应,防治AMI再灌注后无复流。

Objectives To evaluate the effects of sodium ferulate （SF） on no-reflow phenomenon in a rabbit model of acute myocardial infarction （AMI） and reperfusion. Methods Twenty-four mature male rabbits were randomly divided into sham-operated group, control group, sodium ferulate group （25 mg/kg, once 30 rain before coronary artery ligation） with 8 in each group. The later 2 groups were subjected to 4 h of left anterior descending occlusion followed by 2 h of reperfusion. The concentrations of ET-1 in serum, normal, infracted reflow and no-reflow myocardium were determined by radioimmunoassay. The serum concentrations of IL-6 were measured by enzyme linked immunosorbent assay. The area of no-reflow was evaluated with pathological means. Results （ 1 ） Compared with the sham-operated group, concentrations of endothelin-1 （ ET-1 ） in serum in the control group and SF group significantly increased 4 h after occlusion and 2 h after reperfusion （all P 〈 0.01 ） ; Compared before AMI, concentrations of ET-1 in serum in the control group and SF group significantly increased 4 h after occlusion and 2 h after reperfusion （all P 〈 0.01 ） ; Concentrations of ET-1 in serum in SF group were significantly lower than those in control group 4 h after occlusion and 2 h after repeffusion （ all P 〈 0.01 ）. （2） Both in control group and SF group, concentrations of ET-1 in reflow and no-reflow mvocardium significantly increased than those in normal myucardium （all P 〈 0.01 ）. In SF group, the comcentration of ET-1 in reflow myocardium was significantly lower than that in the control group （P 〈 0.01）. （3） Compared with the sham-operated group, concentrations of interleukin-6 （IL-6） in serum in the control group and SF group increased significantly 4 h after occlusion and 2 h after reperfusiun （all P 〈 0.01 ） ; Compared before AMI, concentrations of IL-6 in serum in the control group and SF group signifieantly increased 4 h after occlusion and 2 h after repeffusion （all P 〈 0.01 ） ; Concentrations of IL-6 in senmr in SF group were significantly lower than those in control group 4 h after occlusion and 2 h after repeffusion （all P 〈 0.05 ）. （4） The area of no-reflow of the SF group decreased significantly than that of the control group （ 57.54%±8.36% vs. 83.91% ±5.35% , P 〈 0.01 ）. Conclusions SF is effective in protecting function of endothelium and preventing myocardial no-reflow in rabbits with acute myocardial infarction and repeffusiun.