植物微核技术在监测水质污染中的应用

APPLICATION OF PLANT MICRONUCLEUS TEST TECHNIQUE IN DETECTING WATER POLLUTION

应用韭菜微核技术，检测哈尔滨市量具刃具厂含铬废水的遗传毒性，试验表明：含铬废水可诱发韭菜和蚕豆根尖细胞产生微核，进行回归分析，其蚕豆对Ｃｒ＋６诱变剂回归分析为：ｙ＝２４．９４＋１２７．７７ｘ（ｒ＝０．９９６，Ｐ＜０．０１），韭菜对Ｃｒ＋６诱变剂回归方程为：ｙ＝２１．７４＋１００．２３ｘ（ｒ＝０．９００，Ｐ＜０．０１），韭菜对含铬废水的回归方程为：ｙ＝１１．１７＋３．８３ｘ（ｒ＝０．７８１，Ｐ＜０．０５），韭菜根尖微核技术具有微核效应良好，方法简单，取材容易等优点。

With plant micronucleus test technique, the genetic toxicity of chromium-borne waste water, which is taken from Harbin Measuring Instrument and Cutting Tolls Mill, has been studied. Its result shows that the micronucleus can be, have been, induced by chromium-borne waste water from root-tip cell of Allium tuberosum Roxb. and Vicia faba L. The regression equation between the plants and Cr +6 -inducer is as follows: y=24.94+127.77x(r=0.996, P<0.01) for Vicia faba L. and y=21.74+100.23x(r=0.900, P<0.01) for Allium tuberosum Roxb. It also is Y=11.17+3.83x(r=0.781, P<0.05) between Allium tuberosum Roxb. and chromium-borne waste water. A simple method, being easy of collecting materials, and a good micronucleus effect are shown by micronucleus test technigue on the root-tip cell of Allium tuberosum Roxb.