北京东灵山辽东栎种群DNA多样性的研究

STUDY ON DNA DIVERSITY OF LIAODONG OAK POPULA_TION AT DONGLING MOUNTAIN REGION,BEIJING

在同工酶研究基础上，利用随机扩增多态性ＤＮＡ（ＲＡＰＤ）及ＤＮＡ扩增指纹（ＤＡＦ）方法，从生态学观点分析了北京东灵山区辽东栎（ＱｕｅｒｃｕｓｌｉａｏｔｕｎｇｅｎｓｉｓＫｏｉｄｚ．）种群的遗传结构和多样性。对１２个引物扩增产生的２０５个产物（部分），经Ｓｈａｎｎｏｎ信息指数分析，结果表明：辽东栎种群内部存在丰富的遗传变异，中央种群的遗传多样性高于边缘种群的遗传多样性。总遗传多样性的９５％发生在种群内，只有５％发生在种群间。两种群在个别位点上存在差异。不同年龄组辽东栎的分析结果表明：人为干扰对辽东栎的遗传结构有一定影响。

Random amplified polymorphic DNA(RAPD) and DNA amplification fingerprinting (DAF) markers were used in detecting genetic structure and DNA diversity of two Liaodong oak (Quercus liaotungensis Koidz.) populations at Dongling mountain region, a suburb of Beijing city.Shannon's index of phenotypic diversity was used to partition diversity into components within and between populations. Two hundred and five bands from twelve primers were analyzed. The results showed very high genetic variability within the Liaodong oak populations. The diversity in the central population was higher than that of the marginal one. 95% of total genetic diversity occurred within populations and the coefficient of gene differentiation was 0.05. Significant difference of gene frequency has been detected in a few loci between populations. The study of different age groups of the oak trees implied that deforestation exerted certain impacts on the genetic structure of the Liaodong oak.