摘要
选用20个随机引物，对分自2个分类接种群的8株Frandia纯培养的总DNA进行随机扩增，其中引物OPW15和OPW16能扩增得到较为稳定的RAPD图谱．扩增产物分子量大都分布在0．5～4Kb之间．从稳定的RAPD扩增图谱看，Frankia菌间存在有丰富的遗传多样性；在选定适当引物情况下，能依据共同带将Frankia菌化归为同一分类接种群．
Eight Frankia strains from 2 taxonomic inoculation groups were analysed for their genetic diversity with Random Amplified Polymorphic DNA(RAPD) technique. Twenty random primerswere used, and the stable RAPD patterns could be detected with primer OPW15 and OPW16.The molecular weight of amplification products was between 0. 5 ～ 4Kb, indicating a great genetic diversity withim Frankia strains. Therefore, with proper primer in RAPD analysis, different Frankia strains could be allocated to the same inoculation group, based on commonbands.
出处
《应用生态学报》
CAS
CSCD
1998年第1期59-63,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金!39470005
中国科学院"九五"重大B项目
