摘要
本文利用荧光标记方法测量了刀豆素A、麦芽凝集素、酵母多糖刺激引起的巨噬细胞膜电位、胞浆pH、溶酶体pH的变化。结果显示三种配体均导致细胞膜电位超极化,胞浆pH降低,溶酶体pH升高,三个生理参量趋于稳定时间稍有不同。胞浆pH的降低可能有抑制内吞的作用,溶酶体pH上升是触发溶酶体内容物外排的基本因素。内吞引起的这些变化是细胞代谢过程中自我调节和保护的表现。
The effects of Con A,WGA,Zymosan A on membrane potential,cytosolic pH and lysosomal pH of ctltured mouse macrophages were measured with fluorescence method.The results showed all the three kinds of ligands caused macrophage membrane potential hyperpolarization,cytosolic acidification and lysosomal alkalization,but reached their stabilized conditions at different times.The acidification of cytosl might inhibit furgher endocytosis.Alkalization of lysosomes was a basic factor for exocytosis of lysosomal contents.These receptor-mediated induced changes reflected cellular self-regulation and self-protection.Supported by the National Natural Science Foundation of China(No.3947O347)
出处
《生理学报》
CAS
CSCD
北大核心
1998年第1期111-114,共4页
Acta Physiologica Sinica
基金
国家自然科学基金!No39470347
关键词
巨噬细胞
受体介导内吞
膜电位
胞浆
溶酶
PH
macrophage
receptor-mediated endocytosis
membrane potential
cytosolic pH
lysosomal pH