X射线衍射法在研究蛋白质动态过程中的应用

Using X-Ray Diffraction to Study the Dynamic Process of the Protein

介绍了Ｘ射线衍射技术在研究蛋白质动态过程中的应用．首先介绍了用常规Ｘ射线衍射法和劳埃Ｘ射线衍射法等数据采样法研究反应时间为几分钟的蛋白质催化反应．然后介绍了通过选择不匹配底物，不适宜酸度，选择温度和酸度的跳跃，金属和光化学瞬时激发达到反应的同步来研究反应时间为几秒钟的蛋白质催化反应．

A brief introduction is given about the application of X-ray diffraction to the study of the dynamic process of the protein. Firstly, it gives some description about using traditional and Laue X-ray diffraction to study the dynamic process of a protein in a reaction taking place in a few minutes. Secondly, it is about reaction synchronization used to study the dynamic process of a protein in a few seconds. By choosing unmatchable substrate and unsuitable pH value, by choosing temperature and pH value jumping and by choosing metal ion and photochemical induction, enzyme reaction can be activated instantaneously.