摘要
用常规琼脂糖凝胶电泳ＵＶＢ照射后培养不同时间的ＮＩＨ３Ｔ３细胞ＤＮＡ，两个样本均未出现凋亡梯形带，而用相同条件处理的昆明小鼠胸腺细胞ＤＮＡ出现了典型的梯形带．再用反转电场琼脂糖凝胶电泳（ＦＩＧＥ）ＵＶＢ照射后培养不同时间的ＮＩＨ３Ｔ３细胞ＤＮＡ，发现ＤＮＡ先断裂成低于２３ｋｂ的大分子片段，然后进一步断裂成小分子片段，但始终未出现梯形带，说明ＤＮＡ并不总是从核小体之间断裂的．
When conventional gel electrophoresis was used, it was found that DNA of NIH3T3 cells cultured for different time after UVB irradiation had no DNA ladders, but thymocytes of Kunming mouse processed by the same method had DNA ladder. The results of field inversion electrophoresis showed that after UVB irradiation, the DNA of NIH3T3 cleaved into high molecular weight fragments at first, and then into low molecular weight fragments, still without the appearance of DNA ladders. It is suggested that DNA cleavage of apoptotic cell is not always initiate at internucleosome.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第1期53-56,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
