摘要
将人的红细胞生成素（ＥＰＯ）基因克隆在ＣＭＶ启动子的下游，构建ＥＰＯ表达质粒，在ＢＨＫ２１细胞中表达成功后，将该质粒经脂质体包埋，再导入到小鼠的骨骼肌内，在体内亦获得了表达，一个月时小鼠血浆中人红细胞生成素的表达量高达１３４０ｎｇ／Ｌ。
The expression vector was successfully constructed with the cloning of erythropoietin genomic DNA into the downstream of CMV promoter,and expressed in transfected transiently BHK21 cells. Then it was introduced into the skeletal muscle of mice by the mediation of lipofectin. As a result, the EPO expressed in serum of mice reached highly to 1 340 ng/L in one month.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第1期68-71,共4页
Progress In Biochemistry and Biophysics
