EGF受体和IGF-1受体在人鼻咽癌组织中的表达及临床意义

Expression and Clinical Significance of EGF Receptor and IGF-1 Receptor in Nasopharyngeal Carcinoma

目的:研究表皮生长因子受体(EGFR)和胰岛素生长因子-1受体(IGF-1R)在人鼻咽癌(NPC)组织中的表达及临床意义。方法:应用免疫组织化学SP法检测NPC组织及鼻咽非肿瘤组织EGFR和IGF-1R蛋白以及用RT-PCR法检测EGFR和IGF-1R mRNA的表达。结果:EGFR和IGF-1R蛋白在NPC组织的过表达率分别为65.3%(49/75)和56%(42/75),在鼻咽非肿瘤组织中过表达率分别为0%和14.3%(3/21)。鼻咽癌组与对照组相比,EGFR蛋白表达有统计学意义(P<0.01),鼻咽癌组织中EGFR和IGF-1R蛋白的过表达对不同年龄、性别的患者无统计学差异,但与淋巴结转移、远处转移、复发和5年生存率有相关性(P<0.01或P<0.05)。鼻咽癌组与对照组相比,IGF-1R mRNA和EGFR mRNA表达亦有统计学差异(P<0.01或/P<0.05)。EGFR和IGF-1R两者在鼻咽癌中表达呈正相关(r=0.367,P=0.001)。结论:EGFR和IGF-1R mRNA和蛋白过度表达可能与鼻咽癌的复发和转移有关,检测EGFR和IGF-1R mRNA和蛋白过度表达可作为判断鼻咽癌复发和转移的潜在指标之一。

To investigate the expressions of EGF and IGF-1 receptors and their relation to the growth and apoptosis of nasopharyngeal carcinoma （NPC） and to explore the clinicopathologic significance. Methods： Seventy-five patients with NPC and twenty-one noncancerous nasopharyngeal epithelial samples were retrospectively studied from year 1997 to 2006, and the expressions of EGF and IGF-1 receptors were detected by immunohistochemical staining. Results. The levels of these two proteins significantly correlated with each other （correlation coefficience=0. 367, P =0. 001）. And the overexpression rates of EGF and IGF-1 receptors were 65.3% and 56% in nasopharyngeal samples, respectively. Furthermore, both protein expressions were significantly higher in NPC patients with cervical lymph node or distant metastasis than in NPC patients without. Conehlsion.- These findings demonstrate that both receptor proteins may play important roles in the invasion, metastasis, and recurrence of NPC. Both receptors are valuable markers for assessing the prognosis of NPC.