布地奈德和罗氟司特合用对哮喘小鼠气道树突状细胞的作用

Effect of Pulmicort and Roflumilast on the Airway Intraepithelial Dendritic Cells in Murine Models

目的:研究布地奈德和罗氟司特联合应用对哮喘小鼠肺内树突状细胞(DC)数量的影响。方法:BALB/c小鼠40只随机分5组,即对照组、哮喘组、罗氟司特组、布地奈德组、布地奈德和罗氟司特合用组。采用免疫组织化学染色技术检测小鼠肺、气道DC;计算机图像分析技术对DC进行定量测定。结果:对照组小鼠的气管、支气管、肺泡和脏层胸膜构成了一个NLDC-145+DC网络,正常对照组肺内NLDC-145+DC数量为(60±10)个/mm2上皮面积,哮喘组小鼠肺内NLDC-145+DC数量[(210±35.6)个/mm2]较对照组显著增加(P<0.01);哮喘小鼠经联合组处理后,肺内DC数量明显低于未处理的哮喘组[(64±16.7)个/mm2比(210±35.6)个/mm2,P<0.01],且合用组与单用组比较,肺内DC数量亦显著下降(P<0.01)。结论:下调肺内DC数量可能是布地奈德与罗氟司特治疗哮喘的一个重要机制;布地奈德和罗氟司特合用治疗哮喘既安全又有效,具有潜在的应用前景。

To investigate the effect of pulmicort and roflumilast on the number of airway intraepithelial dendritic cells （DCs） in murine models. Methods： Forty BALB/c mice were randomly and equally divided into five groups： control group, asthma group, roflumilast-treatment group, pulmieort-treatmengt group, and pulmicort ＋ roflumilast treatment group. DCs were stained by immunohistoehemistry and. analyzed by computer. Results： After ovalbumin challenge, the whole amount of dendritic cells in trachea, bronchus and alveolar were revealed by horseradish peroxidase staining using the NLDC-145＋ DC monoclonal antibody. The number of NLDC- 145＋ DC in the bronchial mucosa of asthma mice was more than that in healthy controls （P〈0.01）. After seven days＇ treatment with pulmicort and roflumilast, the number of NLDC-145＋ DC was less than that in asthma group （P〈0.01）, and the number of NLDC-145＋ DC of pulmicort and roflumilast-treatment group was less than that in pulmieort or roflumilast-treatment group （P〈0.01）. Conclusion： Dendritic cells might be involved in the inflammation of airway of asthmatic murine, and pulmicort combined with roflumilast may down-regulate the number of dendritic cells much more than pulmicort or roflumilast solo use.