期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人乳腺癌干细胞中CCR5的表达及意义 被引量：2

Expression of CCR5 in Breast Cancer Stem Cells and Its Significance
原文传递
导出
摘要 目的:探讨人乳腺癌干细胞和乳腺癌细胞中CCR5的表达及意义。方法:采用流式分选技术从人乳腺癌细胞系MCF-7中分选出乳腺癌干细胞,并利用SYBR实时PCR技术测定不同亚群乳腺癌细胞中CCR5的表达。结果:熔解曲线分析和琼脂糖凝胶电泳结果证实PCR反应的特异性;相对定量结果显示,乳腺癌干细胞CD44+CD24-/low的CCR5表达水平为对照组CD44+CD24+乳腺癌细胞的6.14倍(P<0.05)。结论:高表达CCR5的乳腺癌干细胞与乳腺癌细胞相比具有更强的侵袭转移能力,可能是乳腺癌转移关键因素之一。 To detect the expression of CCR5 in breast cancer stem cells and differentiated cells, and to discuss its clinical significance. Methods： The breast cancer stem cells were separated from MCF-7 cells by flow cytometry-based selection on freshly isolated cells. The expressions of CCR5 mRNA in different subpopulations were measured by real-time PCR. The relative quan tification analysis was performed with comparative threshold cycle （△△Ct） method. Results： Melting curve analysis and agarose gel electrophoresis confirmed the specificity of the amplifica tion products. Compared with that in breast cancer differentiated cells, the expression of CCR5 in breast cancer stem cells were significantly higher by 6. 14 folds （P〈0.05）. Conclusion： Compared with bret cancer differentiated cells, breast cancer stem cells might have stronger abilities in invasion and metastasis via over-expression of CCR5 genes, which is one of the key factors for metastasis of breast cancer.
作者 谭成 易长虹 李娟娟 张懿敏 涂毅 王卫星 孙圣荣
机构地区 武汉大学人民医院乳腺甲状腺外科
出处 《武汉大学学报（医学版）》 CAS 北大核心 2009年第3期379-382,共4页 Medical Journal of Wuhan University
关键词 乳腺癌 干细胞 CCR5 侵袭 转移 Breast Cancer Stem Ceils CCR5 Invasion Metastasis
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献13

  • 1Reya T, Morrison SJ, Clarke MF, et al. Stem cells, cancer, and cancer stem cells[J].Nature, 2001, 414 (6 859) : 105-111.
  • 2Yu F, Yao H, Zhu P, et al. Let -7 regulates self renewal and tumorigenicity of breast cancer cells [J].Cell, 2007, 131:1 109-1 123.
  • 3Karnoub AE, Dash AB, Vo AP, et al. Mesenchymal stem cells within turnout stroma promote breast cancer metastasis[J].Nature, 2007, 449 (7 162): 557-563.
  • 4Robinson SC, Scott KA, Wilson JL, et al. Chemokine receptor antagonist inhibits experimental breast tumor growth[J].Cancer Res, 2003, 63(23):8 360-8 365.
  • 5Al-Hajj M, Wicha MS, Benito-Hernandez A, et al. Prospective identification of tumorigenic breast cancer cells[J]. Proc Nati Acad Scie, 2003, 100(7):3 983- 3 988.
  • 6Azenshtein E, Luboshits G, Shina S, et al. The CC chemokine RANTES in breast carcinoma progression: regulation of expression and potential mechanisms of promalignant activity[J].Cancer Res, 2002, 62(4):1 093-1 102.
  • 7王栋,郭满盈,郭葆玉.趋化因子受体5在乳腺浸润性导管癌组织的表达及意义[J].第二军医大学学报,2005,26(6):657-659. 被引量：14
  • 8Youngs SJ, Ali SA, Taub DD, et al. Chemokines induce migrational responses in human breast carcinoma cell Lines[J]. Int J Cancer,1997, 71(2):257-266.
  • 9Tu SM, Lin SH, Logothetis CJ. Stem-cell origin of metastasis and heterogeneity in solid tumours[J]. Lancet Oncol, 2002,3(8) :508-513.
  • 10Croker AK, Allan AL. Cancer stem cells implications for the progression and treatment of metastatic disease [J]. J Cell Mol Med, 2008,12(2):374-390.

二级参考文献11

  • 1Loetscher P,Uguccioni M,Bordoi L,et al. CCR5 is characteristic of Th1 lymphocytes [J].Nature,1998,391(6665):344-345.
  • 2Mack M,Bruhl H,Gruber R,et al. Predominance of mononuclear cells expressing the chemokine receptor CCR5 in synovial effusions of patients with different forms of arthritis[J].Arthritis Rheum,1999,42(5):981-988.
  • 3Karpus WJ,Ransohoff RM.Cutting adge commentary: Chemokine regulation of experimental autoimmune encephalomyelitis: temporal and spatial expression patterns govern disease pathogenesis [J].J Immunol,1998,161(10):2667-2671.
  • 4Choe H,Farzan M,Sun Y,et al. The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates [J].Cell,1996,85(18):1135-1148.
  • 5Müller A,Homey B,Soto H,et al. Involvement of chemokine receptors in breast cancer metastasis [J].Nature,2001,410(6824):50-56.
  • 6Rossi D, Zlotnik A. The biology of chemokines and their receptors [J].Annu Rev Immunol, 2000,18(3): 217-242.
  • 7Frederick MJ,Clayman GL. Chemokines in cancer[J]. Exp Rev Mol Med,2001,18(7):1-18.
  • 8Schrader AJ,Lechner O,Templin M,et al. CXCR4/CXCL12 expression and signalling in kidney cancer [J].Br J Cancer,2002,86(8):1250-1256.
  • 9Mashino K,Sadanaga N,Yamaguchi H,et al. Expression of chemokine receptor CCR7 is associated with lymph node metastasis of gastric carcinoma [J].Cancer Res, 2002,62(10):2937-2941.
  • 10Azenshtein E,Luboshits G,Shina S,et al. The CC chemokine RANTES in breast carcinoma progression: regulation of expression and potential mechanisms of promalignant activity[J].Cancer Res,2002,62(4):1093-1102.

共引文献13

同被引文献19

  • 1王栋,郭满盈,郭葆玉.趋化因子受体5在乳腺浸润性导管癌组织的表达及意义[J].第二军医大学学报,2005,26(6):657-659. 被引量：14
  • 2Loetseher PM, Ugueeioni L, Bordoi M, et al. CCR5 15 character is tie of Thl lymphocytes[ J]. Nature, 1998,391 (6665) :344-348.
  • 3Azenshtein E, Luboshits G, Shina S, et al. The CC chemokine RANTES in breast carcinoma progression:regulation of expression and potential mechanisms of premalignant activity [ J ]. Cancer "Res ,2002,62 (4) : 1093-1102.
  • 4Balkwill F. Chemokine biology in cancer [ J ]. Semin Immunol, 2003,15 ( 1 ) :49-55.
  • 5Muller A, Homey B, Soto H, et al. Involvement of chemokine receptors in breast cancer metastasis [ J ]. Nature, 2001,410 ( 6 824) :50-56.
  • 6Karnoub AE, Dash AB, Vo AP, et al. Mesenchymal stem cells within tumor stroma promote breast cancer metastasis[ J]. Nature, 2007,449 ( 7162 ) : 557 -563.
  • 7Azenshtein E, Luboshits G, Shina S, et al. The CC chemokine RANTES in breast carcinoma progression:regulation of expression and potential mechanisms of premalignant activity [J]. Cancer Res ,2002,62 (4) : 1093-1102.
  • 8Frederick MJ, Clayman GL. Chemokines in cancer[ J]. Exp Rev Mol Med, 2001, 18(7) :1-18.
  • 9Mafies S, Mira E, Colomer R, et al. CCR5 espression influences the progression of human breast cancer in P53-dependent manner [J]. J EXP Med,2003,198(9):1381-1389.
  • 10Degerli N, Yilmaz E, Bardakci F. The delta 32 allele distribution of the CCR5 gene and its relationship with certain cancers in a Turkish population [ J ]. Clin Bioehem. 2005, 38 (3) :248-252.

引证文献2

二级引证文献4

武汉大学学报（医学版）
2009年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部