摘要
采用营养液水培,研究了不同浓度(5?10?20?50?100、130 mg/L)镍处理下小麦幼苗叶片SOD、POD和CAT同工酶谱的变化。结果表明:镍处理下,小麦幼苗叶片SOD3-4、POD6-9、CAT1酶带减弱,SOD1-2、POD1-5诱导增强,镍通过影响SOD和CAT同工酶强弱而影响酶活性,通过改变POD同工酶条数和强弱来影响POD活性。
The effects of Ni^2＋ in different concentration （5, 10, 20, 50, 100 mg/L） on isozymograms of SOD, POD and CAT in wheat （ Triticum aestivum L. ） seedling leaf were studied by the method of hydroponics. The results showed that under nickel treatment, the isozymograms of SOD3 - 4, POD6 - 9 and CAT1 weakened obviously compared with the control, whereas the isozymograms of SOD1 - 2 and POD1 - 5 strengthened obviously. So excess nickel affected SOD and CAT activities in wheat seedling leaf by isozymograms expression, and affected POD activity by isozymogram band numbers and levels.
出处
《安徽农学通报》
2009年第9期47-48,232,共3页
Anhui Agricultural Science Bulletin
关键词
镍
小麦
抗氧化酶
同工酶
浓度
Nickel
Wheat
Antioxidase
Isozyme
Concentration
