裸燕麦胚性愈伤组织培养及悬浮系的建立

The Culture of Embryogenic Calli and the Establishment of Suspension Cell Line of Naked Oats

裸燕麦成熟胚在ＩＮ６培养基上诱导培养，初始愈伤组织为白色、瘤状、外软内硬、易分化植株的非松脆类型。当在ＩＭ１～ＩＭ４培养基上进行循环式调控培养，经７～８个月，初始愈伤组织转变为浅黄色、小颗粒状、生活力强的松脆型胚性愈伤组织。将胚性愈伤组织置于液体培养基中悬浮培养，得到分散性好、生长快的悬浮细胞系。悬浮系经分化培养获得了再生植株，移栽成活率达９５％以上。

The mature embryos of six naked oats varieties ( Avena nuda L.) were cultured to induce callus in IN medium.The frequency of callus formation of variety “GY 8” was the highest (95.4%) among the six genotypes.The initial callus from the mature embryos of naked oats was white ,nodular,outside soft and inside hard,easier differentiated plantlet.This structure was regarded as nonfriable state.To improve its regeneration properties,the callus was cultured in IM 1 ,IM 2 ,IM 3 ,IM 4 media successively.After seven or eight months,embryogenic callus became wish yello white,granular,finely divided,and relatively friable.Frequencies of embryogenic calli were maintained steadily over 95%.However,decline speed of callus vitality was slowed down.The cell suspension cultures were established from friable calli.The plantlets were induced from the suspension cell line in differentiation medium.The survival rate of plantlet transplantation was over 95%.