摘要
本研究以非放射性标记的猪乳铁蛋白(PorcineLactoferin简称PLF)cDNA为探针,通过染色体原位杂交法,对PLF基因进行了染色体定位。实验中采用金胶抗体技术并结合使用银增强系统,提高了方法的灵敏度。利用染色体的组型分析,对杂交点的分布进行统计学分析。52%(26/50)的分裂相在第2号染色体具银粒分布,实验结果表明:PLF基因定位于猪2号染色体2q12区域。
In order to located the PLF gene to porcine chromosome,we use PLF cDNA as the probe which was nonradioactive labeled for in situ hybridization.The method is based on hybridizing digoxigen probe to metaphase chromosome.Digoxigen gold signals are amplified by silver precipitation.The experiment has a higher sensitive.The number of silver grains on every chromosome was calculated and statistical analyzed.The fifty two percent of hybridized metaphases exhibited silver grains at the terminal region of long arm of chromosome 2.The result showed that the PLF gene is located at 2q 12 .
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第1期96-100,共5页
Chinese Journal of Biotechnology
关键词
猪乳铁蛋白
铁蛋白
原位杂交
基因定位
Porcine lactoferrin(PLF),gene localization, in situ hybridization