用原位杂交法定位猪乳铁蛋白基因于染色体2q^(12)

Localization of PLF Gene at Porcine Chromosome 2q 12 Band by In Situ Hybridization

本研究以非放射性标记的猪乳铁蛋白（ＰｏｒｃｉｎｅＬａｃｔｏｆｅｒｉｎ简称ＰＬＦ）ｃＤＮＡ为探针，通过染色体原位杂交法，对ＰＬＦ基因进行了染色体定位。实验中采用金胶抗体技术并结合使用银增强系统，提高了方法的灵敏度。利用染色体的组型分析，对杂交点的分布进行统计学分析。５２％（２６／５０）的分裂相在第２号染色体具银粒分布，实验结果表明：ＰＬＦ基因定位于猪２号染色体２ｑ１２区域。

In order to located the PLF gene to porcine chromosome,we use PLF cDNA as the probe which was nonradioactive labeled for in situ hybridization.The method is based on hybridizing digoxigen probe to metaphase chromosome.Digoxigen gold signals are amplified by silver precipitation.The experiment has a higher sensitive.The number of silver grains on every chromosome was calculated and statistical analyzed.The fifty two percent of hybridized metaphases exhibited silver grains at the terminal region of long arm of chromosome 2.The result showed that the PLF gene is located at 2q 12 .