生物素对地衣芽孢杆菌生长和乳酸形成的影响

Studies on the Formation of Lactic Acid and Growth of the Bacillus licheniformis by Biotin Variation

生物素对地衣芽孢杆菌生长和乳酸形成的影响胡尚勤（重庆师范学院生物系重庆６３００４７）地衣芽孢杆菌（Ｂａｃｉｌｕｓｌｉｃｇｅｎｉｆｏｒｍｉｓ）是我国９０年代首次从正常待产妇阴道拭子分离出来的一个无毒新菌株。现在国内已经用它来生产“整肠生”活菌剂。它是一...

The effect of the biotin variation on the specific growth rate of Bacillus licheniformis and the formation of lactic acid were studied by using vibration culture. The results show that, when the biotin was 0.3%,0.4%,0.5%, the average specific growth rate(μ) is 0.027 that is 1.23 time as much as comparasion(μ=0.021). The highest yield of lactic acid was 12.0μg/ml,11.5μg/ml,10 9μg/ml,that was 18.18%,17.42%,16.52%,as much as comparasion(66.0μg/mL). These results show that the relationship between formation of lactic acid, specific growth rate of B.licheniformis and biotin content,which is a meaningful guide for further fermentation production of this reagent using the bacterium.