神经内科住院患者医院感染肺炎克雷伯菌的β-内酰胺酶分类检测

Study on the Classification of the β-Lactamases Produced by Klebsiella pneumoniae of inpatient in department of neurology

目的了解神经内科住院患者医院感染肺炎克雷伯菌所产各种β-内酰胺酶（β-lase）分布情况。方法采用多底物改良相邻纸片法检测神经内科住院患者医院感染34株肺炎克雷伯菌所产各种β-1ase。结果神经内科住院患者医院感染34株肺炎克雷伯菌中产β-lase菌株27株，总β-Mase检出率为79．4％，其中产青霉素酶11株（32．4％），广谱酶1株（2．9％），单产ESBLs5株（14．7％），产复合酶10株（29．4％），ESBLs总计15株（44．1％），未检出头孢菌素酶、培南酶。结论本组神经内科住院患者医院感染肺炎克雷伯菌产β-laset以青霉素酶、ESBLs为主，未检出头孢菌素酶、培南酶。

Objective To study the classification of the D-Lactamases Produced by Klebsiella pneumoniae of inpatient in department of neurology. Methods detecting the D-Lactamases produced by Klebsiella pneumoniae of inpatient in department of neurology with a multi-disk synergy test. Results The 27 strains of 34 strains produce the β-Lactamases, total produce rate is 79.4%, 11 strains produce penicillinases （32.4%）, 1 strain producing broad-spectrum β-Lactamases （BSBLs,2.9%）, 5 strains produce extented-spectrum β- Lactamases （ESBLs, 14.7%） alone, 10 strains produce AmpCβ-Lactamases and ESBLs （29.4%）, total produce ESBLs rate is 44.1% （15 strains）, all did not produce cephalosporinases and carbapenem- hydrolyzingβ- Lactamases （CHBLs）. Conclusions The types of β-Lactamases produced by the 34 strains are mainly pcnicillinases and ESBLs, all did not produce eephalosporinases and CHBLs.