未知基因p2.18

Unknown Gene P2.18

用根据GRF设计的引物,以猪的下丘脑总RNA和mRNA为模板、进行RT—PCR扩增.将扩增产物纯化并克隆到T载体(pGEM3—Zf),自动测序仪测定其序列.发现所克隆的基因并非GRF基因.应用BLAST软件对其在INTERNET作了分析,未能确定为何种基因.

We designed a experiment to obtain porcine GRF cDNA. Total RNA and mRNA were extracted from porcine hypotheiemus. Primers were designed according to publised porcine GRF cDNA sequence. RT-PCR was carried out to the standard method. PCRproduct was cloned to T-vector. Positive clone was selected and sequenced. The result showed that the cloned cDNA was not GRF gene. We anylyzed the sequence in Internet by Blast, finding no similar.