太湖新银鱼线粒体DNA物理图谱及分析

Physical Map and Analysis of Mitochondrial DNA from Neosalanx taihuensis Chen

采用改进的碱裂解法制备太湖新银鱼线粒体ＤＮＡ，构建其ＰｖｕⅡ、ＢａｍＨＩ、ＸｈｏＩ、ＳａｌＩ、ＥｃｏＲＩ和ＰｓｔＩ等６种限制性内切酶的物理图谱。比较了鲑形目３种鱼的线粒体ＤＮＡ物理图谱，根据限制性位点差异法计算它们之间的遗传距离，利用ＵＰＧＭＡ聚类分析法构建分子聚类图，结果表明：红点鲑属与大西洋鲑先聚在一起，再与太湖新银鱼相聚，前两者间遗传距离为１２２３％，亲缘关系较近，太湖新银鱼与这两者间的遗传距离为２１３６％，亲缘关系较远，表明银鱼科与鲑科分离较早。

The mitochondrial DNA(mtDNA) from Neosalanx taihuensis Chen was prepared by the improved alkaline lysis procedure. The cleavage sites map of six kinds of restriction endonucleases( Pvu Ⅱ, Bam HI, Xho I, Sal I, Eco RI and Pst I) was determined. The physical maps of mtDNAs from N. taihuensis Chen, Salvelinus taxa and Salmo salar were analyzed. Genetic distances between mtDNAs were calculated by the formula of Nei and Li (1979). Phylogenetic reconstruction method, UPGMA, was used to analyze the genetic relationships and the molecular dendrogram was established and revealed: the genetic distance between Salvelinus taxa and Salmo salar is 12.23% and that between N. taihuensis Chen and Salvelinus taxa? 玈. salar is 21.36%. Thisc indicates that Salangidae diverged from Salmoninae earlier.