球孢链霉菌一种抗生素生物合成基因的核苷酸序列分析

NUCLEOTIDE SEQUENCE ANALYSIS OF AN ANTIBIOTIC BIOSYNTHESIS GEM OF STREPTOMYCES GLOBISPORUS

C-1027是一种具有极强抗肿瘤活性的新型抗生素，由球孢链霉菌（Streptomycesglobisporus）产生。F2DNA片段含有一种编码C－1027生物合成的基因。以质粒pUC18为载体，对其进行了亚克隆，并对该片段进行了核苷酸序列分析，发现一编码122个氨基酸的开放阅读框架，经检索可能是一种新的序列。

Antitumor antibiotic C-1027 produced by Streptomyces globisporus has veryhigh biological activity both in vivo and in vitro. Researeh works showed that one ofbiosynthesis gene of C-1027 is in the F2 DNA fragment The plasmid pUC18 wasused as vector to subelone the ro DNA fragment. The nucleotide sequence analysisfor F2 DNA fragment was carried out. Results showed that there is an open readingframe encoding for 122 amino acids. According to EMBO and GeneBanks data, thissequence may be a new one.