从须纤恙螨分离到恙虫病立克次体的研究

First Isolation of Rickettsia Tsutsugamushi from Leptotrombidium Palpalis

目的：研究纤须恙螨的恙虫病病原学意义及潜在媒介作用。方法：以７１～１５０只左右须纤恙螨为一批接种小白鼠，共分４批。结果：均成功地分离到恙虫病立克次体。传代小鼠的临床症状有弓背、耸毛、厌食等，内脏仅见肝脏肿大，脾脏只在最初２代有个别肿大。ＩＦＡ法检测传代阳性小鼠血清中的恙虫病立克次体ＩｇＧ抗体，均呈阳性反应，血清分型结果均为Ｇｉｌｉａｍ型。对须１１０４株ＬＤ５０测定结果为１０－１４３７５。结论：须纤恙螨是仅作为恙虫病立克次体的宿主，抑或既是宿主又可充当潜在媒介，在恙螨→人或恙螨→鼠之间起作用。

This article reported the isolation of Rickettsia tsutsugamushi (Rt.) from Leptotrombidium palpalis in China for the first time. 4 strains were obtained from 4 groups of chiggers (71-150 chiggers as a group). Enlargement of liver was observed in experimented mice inoculated with isolates, but the spleen′s enlargement was rarely observed. Serological identification of 4 strains revealed strong positive reaction with Rt. (Gilliam strain). More research was needed to confirm whether L.palpalis can act as a vector of Rt. from chiggers to rodents or from chiggers to men.