摘要
目的检测Dickkopf1(DKK1)在人胶质瘤细胞株MGR3和UWR7中的表达情况。方法采用酶联免疫吸附实验,逆转录多聚酶链反应等方法对DKK1在人胶质瘤细胞株MGR3和UWR7中表达水平进行检测。结果酶联免疫吸附实验提示DKK1在MGR3和UWR7中均呈高浓度;逆转录多聚酶链反应提示DKK1基因在胶质瘤细胞株MGR3和UWR7中高表达,但在正常脑组织中表达不明显。结论DKK1在人胶质瘤细胞株MGR3和UWR7中高表达,提示DKK1可能在胶质瘤中的发生发展中起重要作用。
Objective To detect the expression of dickkopf1 ( DKK1 ) in human glioma cell lines MGR3 and UWR7. Methods The expression of DKK1 in human glioma cell lines MGR3 and UWR7 was detected by enzyme-linked immunosorbant assay (ELISA) and reverse transcriptase-polymerase chain reaction (RTff PCR). Results ELISA demonstrated that the expression of DKK1 was both up-regulated in MGR3 and UWR7. RT-PCR showed that DKK1 transcripts was over-expressed in MGR3 and UWR7, which was absent in normol brain tissues. Conclusion DKK1 is over-expressed in glioma cell lines MGR3 and UWR7, which may play an important role in the initiation and development of glioma.
出处
《中国临床实用医学》
2009年第3期3-4,共2页
China Clinical Practical Medicine
基金
江苏省卫生厅重大项目(项目编号:K200508)