温阳益气平喘方对支气管哮喘大鼠病理形态学的影响及一氧化氮和内皮素的调节作用

Effect of Wenyang Yiqi Pingchuan Recipe on Pathomorphology of Lung and Its Regulation on Lung Tissue Contents of Nitric Oxide and Endothelin-1 in Rats with Bronchial Asthma

目的观察温阳益气平喘方对支气管哮喘大鼠肺组织病理形态学的影响及一氧化氮(NO)和内皮素(ET-1)的调节作用。方法60只SD大鼠随机分为6组,即正常组、模型组、温阳益气平喘高剂量组、温阳益气平喘低剂量组、桂龙咳喘宁组及氨茶碱组,每组10只。除正常组外,以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,给药4周后处死大鼠,观察肺组织NO和ET-1含量和病理组织学变化。结果与正常组比较,模型组、低剂量组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞以及肺组织NO、ET-1含量明显增加,差异有统计学意义(P<0.01);与模型组比较,各治疗组支气管管壁和平滑肌厚度、嗜酸性粒细胞以及肺组织NO、ET-1含量均显著降低,差异均有统计学意义(P<0.05或P<0.01);且高、低剂量组明显低于桂龙咳喘宁组,差异均有统计学意义(P<0.05或P<0.01);与氨茶碱组比较,高剂量组嗜酸性粒细胞浸润减少,差异有统计学意义(P<0.01),NO、ET-1差异无统计学意义(P>0.05)。结论温阳益气平喘方可减少嗜酸性粒细胞浸润,降低肺组织NO和ET-1含量,抑制支气管哮喘大鼠气道炎症和气道重塑等病理过程。

Objective To observe the effect of Wenyang Yiqi Pingchuan Recipe （WYPR） on the pathomorphology of lung and its regulation on lung tissue contents of nitric oxide （NO） and endothelin-1 （ ET-1 ） in rats with bronchial asthma. Methods Sixty SD rats were randomly divided into 6 groups： the normal group, the model group, and the four treated groups treated with high dose WYPR, low dose WYPR, Guilong Kechuanning Capsule and aminophylline, respectively, 10 in each group. Except those in the normal group, all rats of bronchial asthma model were established by egg protein sensitization and provocated by inhalation. The treatments were given via gastrogavage every day starting from the first provocation （the 3rd week of modeling） to the execution. Rats were sacrificed after 4-week treatment, their lung was taken for determining the contents of NO and ET-1, and histopathological changes in lung were observed as well. Results Compared with the normal group, the contents of NO and ET-1 in the lung tissue, the thickness of bronchus wall and bronchus smooth muscle, the number of eosinophil granulocytes increased in the model group and the low dose WYPR group, showing statistical significance （P〈0.01）. As compared with the model group, all the above-mentioned indices were lower in all the 4 treated groups （P 〈0.05 or P 〈0.01 ）, but the lowering in the WYPR treated groups （either high or low dose） was more significant than in the Guilong Kechuanning treated group （P〈0.05 or P〈0.01 ）; while compared with the aminophylline treated group, the high dose WYPR group was superior in reducing eosinophile granulocytes （ P 〈0.01 ）, but no significance between them was shown in NO and ET-1 levels （ P 〉0.05）. Conclusion WYPR could reduce the eosinophilic infiltration, decrease the contents of NO and ET-1 in the lung tissue, restrain the air passage inflammation and inhibit the pathological process as airway remodeling.