6种有机磷农药分离及水中残留高效薄层析测定方法研究

Study on Separation of 6 Kinds of Organophosphorous Pesticides and Determination of Their Residues in Water by HPTLC

[目的]比较有机磷农药的分离方法，用高效薄层色谱测定农药在水中残留。[方法]采用高效薄层析技术AMD和双槽展缸上行展开方式分离水中辛硫磷、毒死蜱、双硫磷、甲基异柳磷、喹硫磷和三唑磷6种有机磷农药，并对自来水中4种有机磷残留的测定方法进行研究。[结果]两种展开方式均能很好分离6种农药。AMD展开Rf值分布范围较窄（相差0.34），双槽展缸上行展开Rf值分布范围较宽（相差0.76）；AMD多步展开检测灵敏度较双槽展缸上行展开低。采用双槽展缸上行展开薄层色谱法检测水中有机磷农药残留，添加回收率在86.05%-109.80%之间，添加回收率、变异系数均满足农药残留分析的要求。[结论]该研究中，双槽展缸上行展开表现出灵敏度较高、操作简便、分析速度快的特点。

[ Objective ] The aim was to compare the separating methods of 6 organophosphorous pesticides and determine their residues in water by HPTLC. [ Method ] 6 kinds of organophosphorous pesticides of phoxim, chlorpyrifos, temephos, isofenphos-methyl, quinalphos and triazophos in water were separated by AMD and ascending development method using two-trough chamber, and the determination method of the residues of the 4 kinds of organophosphorous pesticides from water were studied. [ Result] 2 developing methods all could separate the 6 kinds of organophosphorous pesticides better. The distribution range of Rf value of AMD developing method was narrower （ difference was 0.34 ） and that of ascending development method using two-trough chamber was wider （ difference was 0.76 ）. The sensitivity determined by AMD multistep expansion method was lower than that by ascending development method using two-trough chamber. Using ascending development in twotrough chamber-TLC method determining organophosphorous pesticide residue, the fortified recovery was between 86.05% and 109.80%, then the fortified recovery and variation coefficient all could meet the demand for pesticide residue analysis. [ Conclusion ] In this study, the sensitivity of ascending development using two-trough chamber was higher, and its operation was convenient and analysis speed was rapid.