烟草叶片愈伤组织诱导及分化培养的验证

Verification on the Callus Induction and Differentiation Culture of Tobacco Leaves

[目的]研究烟草愈伤组织的发育情况及其丛生芽或分化点的形态建成。[方法]在无菌条件下,把烟草叶片切块培养在含有2mg/L NAA和1 mg/L 6-BA的MS培养基上诱导愈伤组织的形成;而后,转移愈伤组织到含有NAA 0.1 mg/L+BA 2.0 mg/L的分化培养基上使其产生丛生芽或分化点。[结果]诱导培养20 d后,愈伤组织基本形成且没有发生污染,烟草叶片愈伤组织的诱导培养是成功的。愈伤组织的整体分化率比较高,愈伤组织上形成的丛生芽或分化点比较多,且分布比较均匀;编号为4的分化培养基上愈伤组织的分化不理想,且其丛生芽或分化点分布极不均匀。在烟草叶片愈伤组织的分化培养中NAA和6-BA的添加量分别为0.1和2.0 mg/L。[结论]该研究为植株繁育提供了良好的技术支持。

[ Objective ] The aim was to study the developmental condition of tobacco cal]us and the morphogenesis of its multiple shoots or differentiation points. [ Method] Under aspetic condition, the tobacco leaves were cut into pieces and cultured on MS media with 2 mg/L NAA and 1 mg/L 6-BA for callus induction, and then the ealli were transferrred onto the differentiation media with NAA 0. 1 mg/L ＋ BA 2.0 mg/L for multiple shoot or differentiation point generation. [ Result] After induction culture for 20 d, the calli were basically formed without pollution occurred, so the induction culture of calli from tobacco leaves was successful. The overall differentiation rate of calli was relatively high, there were relatively many multiple shoots or differentiation points formed on calli and they were distributed evenly relatively. The differentiation of callus on the differentiation medium numbered as 4 was non-ideal and the distribution of its multiple shoots or differentiation points was very uneven. The addition amounts of NAA and 6-BA in the differentiation culture of calli from tobacco leaves were 0.1 and 2.0 mg/L resp.. [ Conclusion] The study provided good technical support for plant propagation. Tobacco