皂角刺提取物对荷瘤小鼠肿瘤生长及细胞因子的影响

Effects of Extractive from Spina Gleditsiae (ESG)on Tumor Growth and Cytokines in Tumor-bearing Mice

目的探讨皂角刺提取物对荷瘤小鼠肿瘤生长及细胞因子水平的影响。方法建立小鼠肉瘤S180及小鼠肝癌H22移植性肿瘤模型,观察皂角刺提取物对荷瘤小鼠肿瘤生长的抑制作用;采用ELISA法测定白介素-2(IL-2)、白介素-6(IL-6)、白介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)的水平,探讨皂角刺提取物对荷瘤小鼠细胞因子的影响。结果皂角刺提取物在50、100mg/kg时对S180的生长抑制率分别为44.59%、53.38%,对H22生长抑制率分别为39.88%、46.63%,且能提高荷瘤小鼠的脾指数和胸腺指数;皂角刺提取物能提高荷瘤小鼠细胞因子IL-2、IL-6、IL-12、TNF-α的表达水平。结论皂角刺提取物有较强的体内抗肿瘤活性,且能促进细胞因子的表达,增强机体免疫功能。

Objective To study the effects of Extractive from Spina Gleditsiae （ESG）on the tumor growth and cytokines in tumor-bearing mice. Methods Antitumor activity of ESG in vivo was observed by the models of implanted H22 or S180 cell line in mice. The expression of TNF-α. IL2,IL-6 and IL-12 in tumor-bearing mice was detected by ELISA assay. Results At dosage of 50. 100 mg·kg^-1, inhibitory rate of ESG is 44. 59% and 53.38% for S180, 39. 88% and 46. 63% for H22, respectively. ESG can increase the expression of TNF-α.IL-2.IL-6 and IL-12. Conclusion ESG exhibits anti-tumor effect obviously in vivo and enhances immune function by promoting the expression of cytokine.