MMP-2 mRNA及蛋白的表达在原发性肝细胞癌组织中的意义

Expression and Significance of Matrix Metalloproteinase-2 mRNA and Protein in Primary Hepatocellular Carcinoma

目的探讨原发性肝细胞癌(HCC)组织中基质金属蛋白酶-2(MMP-2)mRNA和MMP-2蛋白的表达,及其与临床病理参数之间的关系。方法应用RT-PCR技术、免疫组织化学MaxvisonTM法,分别检测47例HCC组织及其癌旁组织、20例正常肝组织中MMP-2 mRNA和MMP-2蛋白的表达情况。结果(1)MMP-2 mRNA和MMP-2蛋白在肝癌中的表达分别为70.2%(33/47)和63.8%(30/47),均高于癌旁组织12.7%(6/47)和正常组织10%(2/20),差异有统计学意义(P<0.01),癌旁组织和正常组织中MMP-2的表达差异无统计学意义(P>0.05);MMP-2 mRNA和MMP-2蛋白在肝癌中表达的两种检测方法差异无统计学意义(P>0.05);(2)癌组织中MMP-2的表达在TNM分期Ⅲ～Ⅳ期组中的阳性表达率高于Ⅰ～Ⅱ期组(P<0.05),血管内有肿瘤栓组MMP-2阳性率明显高于无瘤栓组(P<0.05),有转移组的阳性率高于无转移组(P<0.05)。结论MMP-2的表达与HCC的TNM分期、血管内有无瘤栓和转移密切相关,可作为判断HCC侵袭性的评估指标之一。

Objective To investigate the expression of matrix metalloproteinase-2 （MMP-2） at mRNA and protein levels in primary hepatocellular carcinoma（HCC） and their relationship with clinicopathological parameters. Methods RT-PCR technique and MaxvisionTM immunohistochemical method were used to detect MMP-2 mR-NA and protein levels in 47 paired HCC and cancer adjacent tissue samples and 20 normal liver tissues samples, respectively. Results The positive rates of expressions of MMP-2 mRNA and protein in 47 HCC cases were 70. 2% （33/47）and 63. 8% （30/47）, respectively, which were significantly higher than those in cancer adjacent tissues and in normal liver tissues （12. 7% and 10. 0%, respectively）. The positive rates of MMP-2 expression in HCC were higher than that in adjacent non-HCC liver tissue and in normal liver tissue and the difference was statistically significant （P〈0. 01） ; the difference of MMP-2 expressions between adjacent non-HCC liver tissue and normal liver tissue had no statistically significance （P〉0. 05）. The two methods had no statistically significance （P〉0. 05）. ②The high positive rates of MMP-2 in TNM stages of Ⅲ-Ⅳ were significantly higher than those in stages of Ⅰ-Ⅱ（P〈0. 05）, and the positive rates of MMP-2 in HCC with metastasis was higher than that without metastasis（P〈0. 05）. The positive rate of MMP2 in HCC with tumor thrombus was higher than that without thrombus （P〈0. 05）. Condusion The expressions of MMP-2 are closely correlated with TNM staging, tumor thrombus and metastasis in HCC which may serve as predictors for determining tumor aggressive behavior in HCC.