苞叶杜鹃离体培养及种质试管保存体系的建立

In vitro culture and in vitro germplasm preservation system of Rhododendron redowskianum Maxim.

以苞叶杜鹃新生嫩芽为外植体,应用均匀设计法筛选其最适合的培养基,建立了苞叶杜鹃的离体培养和种质试管保存体系。结果表明:适合苞叶杜鹃基部直接再生芽苗的诱导培养基为DR+TDZ(1.80 mg/L)+IBA(0.05 mg/L),此条件下芽苗分化率为100%;生根培养基为MS(改良)+IBA(0.08 mg/L)+NAA(0.02 mg/L)+KT(0.15 mg/L),这一条件下芽苗生根率达99%以上;试管保存培养基为1/7MS+B9(1.30 mg/L)+根皮苷(2.80 mg/L),常温条件下保存时间超过35个月。以苞叶杜鹃再生植株茎节为材料进行快繁的结果表明,在35 d的培养周期内,平均增殖倍数超过55倍。

The tender buds of Rhododendron redowskianum Maxim. were used as explants and suitable medium compositions were screened through uniform design experiments. The results showed that DR ＋TDZ（ 1.80 mg/L） ＋ IBA（0.05 mg/L） was the most suitable for shoots regeneration immediately at base of tender buds with a regeneration rate of 100%. MS（modified） ＋ IBA（0.08 mg/L） ＋ NAA（0.02 mg/L） ＋ KT（0.15 mg/L） was most suitable for rooting with a rooting rate of 99%. 1/7MS ＋ B9 （1.30 mg/L） ＋ Phloridzin（2.80 mg/L） was most suitable for in vitro germplasm preservation under which the plant materials could be maintained for 35 months at normal temperature. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 55-fold proliferation rate was achieved within 35 days. In vitro culture and in vitro germplasm preservation system of Rh. redowskianum Maxim. has been successfully established in this study.