广州地区2008年手足口病病原体检测分析

Analysis and pathogenic diagnosis of the hand foot and mouth diseases in Guangzhou 2008

目的对2008年广州地区手足口病病例进行病原体检测。方法临床诊断为手足口病病例的粪便、咽拭子或肛拭子标本752份,首先采用荧光定量PCR筛选出总肠道病毒阳性的标本,再分别使用CA16和EV71型的特异引物进行RT-PCR检测。结果总肠道病毒阳性率为8.78%,CA16阳性率为2.26%,EV71阳性率为2.53%。阳性率较高的地区为人口密集区,年龄为2～5岁,男性略多于女性,接触者的阳性率约为患者的一半。结论分子生物学方法可用于手足口病的病原体检测、EV71和CA16分型以及接触者的排查,有利于感染者的早发现、早隔离,对手足口病的监测及防控具有重要作用。

Objective To analyze the pathogenic diagnosis of the hand foot and mouth diseases in Guangzhou 2008. Methods 752 specimens including stool, pharynx swabs and anal swabs from clinically - diagnosed hand food and mouth diseases patients were collected. Panenterovirus was detected by flueorecent PCR, and Coxsaekie A16 and Enterovirus 71 were determined by RT - PCR with species - special primers. Results Positive rate of pan - enterovirus, Coxsackie A16 and Enterovirus 71 were 9.79% ,2.92% and 3.44% , respectively. High positive rate was found in high population area, 2 -5 year old children and male patients. Positive rate of contactors was about the half of that of the patients. Conclusion Molecular biological methods can be used for pathogenic diagnosis of the hand foot and mouth diseases, subtyping of Coxsackie A16 and Enterovirus 71, and for investigation of contactors. It plays an important role in the surveillance and control of the hand foot and mouth diseases.