Artesunate对K562细胞增殖作用及细胞周期的影响

Effect of Artesunate on Proliferation and Cell Cycle in Human Leukemia K562 Cells

目的:观察Artesunate对人白血病细胞株K562细胞增殖及细胞周期的影响。方法:用Artesunate处理K562细胞,细胞计数法绘制生长曲线;MTT比色法检测细胞增殖抑制的效果;倒置光显微镜观察细胞形态学变化;流式细胞术(flowcytometry,FCM)进行细胞周期分析。结果:Artesunate的浓度为1×10-4、1×10-5、1×10-6mol/L时,细胞生长受到显著抑制,并呈剂量依赖性;对K562细胞的半数抑制浓度为3.75×10-6mol/L;倒置光显微镜观察细胞出现不同程度的皱缩,核分裂相减少,内可见空泡,培养基中可见较多分泌颗粒;流式细胞仪检测G2期细胞比例增高。结论:Artesunate对K562细胞生长及增殖有抑制作用,其作用机制可能是通过抑制DNA合成,使细胞增殖停留在G2/M期来实现的。

Objective：To investigate the effects of artesunate on proliferation and cell cycle in human leukemia K562 cells. Methods： Human leukemia K562 cell lines were treated by artesunate. The cell growth curve was drawn according to cell counts. The inhibitory effect on ceil proliferation was assessed by MTT assay. The morphology changes of the nucleons were ob- served under invert microscope. The level of cell-cycle was determined by flow cytometry （FCM）. Results： When the concen- trations of artesunate were 1 1×10^-4,11×10^-5 and 1×10^-4 mol/L, the growth of cells was inhibited remarkably in a dose-dependent manner. The IC50 values of artesunate were 3.751×10^-4 mol/L. It was seen that ceils in different degrees of shrinkage, reduction of nuclear fission, and secretory granules in culture medium under the invert microscope. Flow cytometric analysis showed that ceils were increase in G2 phase. Conclusion： Artesunate can inhibit the growth and proliferation effectively in K562 cells. The inhibitory mechanisms on proliferation inhibition may be reducing DNA synthesis and arresting cell cycle progression in G2/M phase.