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siRNA体内导入策略

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摘要 以small interfering RNA（siRNA）为基础的RNA干扰（RNA interfering，RNAi），是指当与内源性mRNA编码区某段序列同源的双链RNA（dsRNA）导入细胞后，该mRNA发生特异性降解，而导致该基因表达的沉寂。是一种dsRNA分子在mRNA水平上关闭相应序列基因的表达或使其沉默的过程，
作者 章清华 方佩华
机构地区 天津医科大学总医院核医学科
出处 《天津医药》 CAS 北大核心 2009年第5期424-426,共3页 Tianjin Medical Journal
关键词 RNA 小分子干扰 RNA干扰 载体 综述
分类号 R394 [医药卫生—医学遗传学] R454 [医药卫生—治疗学]
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参考文献23

  • 1Santel A, Aleku M, Keil O, et al. A novel siRNA-lipoplex technology for RNA interference in the mouse vascular endothelium[J]. Gene Ther,2006,13(16): 1222-1234.
  • 2vande Water FM, Boerman OC, Wouterse AC, et al. Intravenously administered short interfering RNA accumulates in the kidney and selectively suppresses gene function in renal proximal tubules [J]. Drug Metab Dispos, 2006,34(8): 1393-1397.
  • 3Layzer JM, McCaffrey AP, Tanner AK, et al. In vivo activity of nuclease-resistant siRNAs[J]. RNA, 2004,10(5):766-771.
  • 4Kim SH, Jeong JH, Lee SH, et al. PEG conjugated VEGF siRNA for antiangiogenic gene therapy [J]. J Control Release, 2006,116 (2): 123-129.
  • 5Matsui Y, Kobayashi N, Nishikawa M ,et al. Sequence-specific suppression of mdr1a/1b expression in mice via RNA interference[J]. Pharm Res, 2005 , 22(12): 2091-2098.
  • 6Urban-Klein B, Werth S, Abuharbeid S, et al. RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo[J]. Gene Ther,2005,12(5):461-466.
  • 7Takeshita F, Minakuchi Y, Nagahara S, et al. Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo [J]. Proc Natl Acad Sci USA, 2005, 102 (34):12177- 12182.
  • 8Hassan A, Tian Y, Zheng W, et al. Small interfering RNA-mediated functional silencing of vasopressin V2 receptors in the mouse kidney [J]. Physiol Genomics, 2005,21(3): 382-388.
  • 9Santel A, Aleku M, Keil O, et al. RNA interference in the mouse vascular endothelium by systemic administration of siRNA- lipoplexes for cancer therapy [J]. Gene Ther, 2006,13 (18):1360- 1370.
  • 10Landen CN Jr, Chavez-Reyes A, Bucana C, et al. Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery[J]. Cancer Res, 2005,65( 15): 6910-6918.

二级参考文献12

  • 1安立峰,董震.RNA干扰——肿瘤研究的新工具[J].中华肿瘤杂志,2005,27(7):385-388. 被引量：38
  • 2Shinkaruk S, Bayle M, lain G, et al. Vascular endothelial cell growth factor (VEGF) , an emerging target for cancer chemotherapy[J]. Curr Med Chem Anticancer Agents, 2003, 3 (2) : 95 - 117.
  • 3Masood R, Cai J, Zheng T, et al. Vascular endothelial growth factor (VEGF) is an autocrine growth factor for VEGF receptor-positive human tumors[J]. Blood, 2001,98(6) : 1904-1913.
  • 4de Jong JS, van Diest PJ, van der Valk P, et al. Expression of growth factors, growth-inhibiting factors, and their receptors in invasive breast cancer, Ⅱ: Correlations with proliferation and Angiogenesis [ J ]. J Pathol, 1998, 184( 1 ) : 53 -57.
  • 5Kasukabe T, Okabe-Kado J, Kato N, et al. Effects of combined treatment with rapamyein and eotylenin A, a novel differentiation-inducing agent, on human breast carcinoma MCF-7 cells and xenografts [ J ]. Breast Cancer Res, 2005, 7(6): R1097- 1110.
  • 6Ferrara N. VEGF: an update on biological and therapeutic aspects [J]. Curt Opin Biotechnol, 2000, 11(6) : 617 -624.
  • 7Lacal PM, Ruffini F, Pagani E, et al. An autocrine loop directed by the vascular endothelial growth factor promotes invasiveness of human melanoma cells[ J]. Int J Oncol, 2005, 27(6) : 1625 - 1632.
  • 8Dorsett Y, Tuschl T. siRNAs: applications in functional genomics and potential as therapeutics [ J ]. Nat Rev Drug Discov, 2004, 3 (4) : 318 -329.
  • 9Layzer JM, McCaffrey AP, Tanner AK, et al. In vivo activity of nuclease-resistant siRNAs[J]. RNA, 2004, 10(5): 766-771.
  • 10Schiffelers RM, Ansari A, Xu J, et al. Cancer siRNA therapy by tumor selective delivery with ligand-targeted sterically stabilized nanoparticle[J]. Nucleic Acids Res, 2004, 32(19) : 149.

共引文献12

天津医药
2009年 第5期

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