抑癌基因TIP30在甲状腺乳头状癌中的表达与临床意义

Expression and Clinical Significance of Tumor Suppressor Gene TIP30 in Papillary Thyroid Carcinoma

目的观察抑癌基因Tat作用蛋白30(TIP30)基因在甲状腺乳头状癌中的表达,并分析其在甲状腺乳头状癌临床治疗中的意义。方法选取30例病理蜡块标本,均来自我院2003～2006年期间临床确诊的甲状腺乳头状癌患者,其中男7例,女23例;年龄15～70岁,平均44.7岁;6例为结节性甲状腺肿局部区域癌变(乳头状),2例有甲状腺包膜浸润;病理证实均未发生远处淋巴结转移及病灶周围甲状腺组织的转移。每例标本均分为癌组织和癌旁组织(距肿瘤1～2cm以远并经病理证实的非癌组织),用免疫组织化学法检测标本中TIP30蛋白的表达。用染色指数和平均吸光度值2个指标表示TIP30蛋白的表达量。结果TIP30蛋白主要表达于细胞膜与细胞质中,呈棕黄色颗粒。①染色指数:TIP30蛋白在癌旁组织中有21例(70.0%)呈阳性(积分>2分),9例(30.0%)呈阴性(积分≤2分);在癌组织中其表达减少或缺失,11例(36.7%)呈阳性(积分>2分),19例(63.3%)呈阴性(积分≤2分);两者间比较差异有统计学意义(P<0.05);而癌组织中TIP30蛋白的表达与患者的年龄及性别无关(P>0.05)。②TIP30蛋白在癌组织中表达的平均吸光度值明显低于癌旁组织(P<0.05)。结论初步表明TIP30蛋白在甲状腺乳头状癌中表达减少或缺失,为以后甲状腺乳头状癌的基因治疗提供相关的理论支持。

Objective To investigate the expression of tumor suppressor gene Tat interacting protein 30 （TIP30） gene in papillary thyroid carcinoma and it＇s clinical significance in treatment of thyroid carcinoma. Methods Thirty cases of pathological specimens wax pieces of papillary thyroid carcinoma from 2003 to 2006 in our hospital were selected, in which there were 7 male, 23 female; and the age was from 15 to 70 years old, average 44.7 years. Six cases were nodular goiter with carcinomatous change in local area （papillary）, 2 cases were thyroid capsular in- vasion. Distant lymph node metastasis and lesions surrounding the thyroid tissue were not confirmed by pathology. Every specimen was divided into tumor tissue and adjacent tissue （1--2 cm far away from tumor and non-cancerous tissue was confirmed by pathology）. The expression of TIP30 in specimen was detected by immunohistochemical method with staining index and the average absorbance. Results TIP30 was expressed in the cell membrane and cytoplasm, which was showed as brown particles. ①Staining index： TIP30 in adjacent tissues was expressed highly with 21 （70.0%） positive cases （〉2 points） and 9 （30.0%） negative cases （42 points）, while its expression in cancer tissues was reduced or missed with 11 （36.7%） positive cases （〉2 points） and 19 （63.3%） negative cases （≤2 points）. There was a statistical difference between them （P〈0.05）, and it was not related to age and gender of patients （P〉0.05）. ②The average absorbance of TIP30 in cancer tissues was significantly lower than that in adjacent tissue （P〈0.05）. Conclusion The expression of TIP30 in papillary thyroid carcinoma is reduced or deleted, which can supply some theory support for its gene therapy.