MCP-1、VEGF在胃癌中的表达与幽门螺旋杆菌感染的关系

Relationship Between Expressions of MCP-1 and VEGF in Gastric Cancer and Helicobacter Pylori Infection

目的探讨胃癌和正常胃黏膜组织中单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达水平与幽门螺旋杆菌(helicobacterpylori,HP)感染情况的关系。方法采用免疫组化SP法检测57例胃癌手术标本和相应的正常胃黏膜组织中MCP-1和VEGF的表达水平,改良Giemsa染色法检测HP感染情况。结果胃癌组织中MCP-1和VEGF的表达水平明显高于正常胃黏膜组织(P<0.05)。MCP-1及VEGF在胃癌组织中的表达与性别及年龄无关(P>0.05);MCP-1及VEGF在肿瘤直径>5cm、低分化、有淋巴结转移、有远处转移、TNM分期较高(Ⅲ+Ⅳ期)者中的表达水平明显高于肿瘤直径≤5cm、高或中分化、无淋巴结转移、无远处转移和TNM分期较低(Ⅰ+Ⅱ期)者,差异均有统计学意义(P<0.05)。无论在胃癌组织还是正常胃黏膜组织中,HP感染阳性者其MCP-1和VEGF的表达与HP感染阴性者间的差异均无统计学意义(P>0.05)。结论MCP-1及VEGF在胃癌组织中高表达,与胃癌血管生成及浸润、转移有关;HP感染和胃癌的浸润、远处转移等生物学行为可能无关。

Objective To investigate the expressions of monocyte chemoattractant protein 1 （MCP-1） and vascular endothelial growth factor （VEGF） in gastric cancer tissues and normal gastirc mucosa tissues and the situation of helicobacter pylori （HP） infection, and detect their relationships and clinicopathologic significances. Methods Expressions of MCP-1 and VEGF were detected by immunohistochemistry in gastric cancer tissues and normal gastric mucosa tissues （5--10 cm from the mass）, and HP was detected in specimen from gastric antrum by Giemsa dyeing method. Results MCP-1 and VEGF expressions in gastric cancer tissues were significantly higher than those in normal gastric mucosa tissues （P〈0.05）, but there was no difference in HP positive and negative tissues included the cancer and the normal tissues （P〉0. 05）. The expressions of MCP-1 and VEGF in carcinoma with tumor diameter 〉5 cm, poorly differentiated, lymph node metastasis, distant metastasis and Ⅲ ＋ Ⅳ stage of TNM were significantly higher than those with tumor diameter ≤5 cm, well and moderately differentiated, non-lymph node metastasis, non-distant metastasis and Ⅰ＋ Ⅱ stage of TNM （P〈0.05）. Conclusion The high expressions of MCP-1 and VEGF in gastric cancer may relate to tumor angiogenesis and metastasis, but HP infection may be irrelevant.