广西壮族人群NSCLC中PTEN基因表达的研究

Study on expression of PTEN in non-small cell lung cancer in Guangxi Zhuang people

目的探讨广西壮族人群NSCLC中PTEN表达情况及其与临床病理因素的相关性。方法采用逆转录-聚合酶链反应(RT-PCR)检测34例壮族人群肺癌组织和14例正常肺组织中PTEN基因表达。结果肺癌组织中PTEN基因表达率35.0%(12/32),正常肺组织表达92.8%(13/14),两组中PTEN基因表达率差异有统计学意义(P<0.001)。PTEN基因缺失与肺癌的病理类型、分化程度有关。结论NSCLC中存在较高PTEN基因表达缺失,表明PTEN基因在肺癌的发生发展过程中起重要作用。

Objective To investigate the expression of suppressor gene FrEN （phosphatase and tensin homology deleted on chromosome ten） and its correlation with elinicopathologieal factors in non-small cell lung cancer （NSCLC） of Zhuang people. Methods The PTEN mRNA were amplified by reverse transcription polymerase chain reaction （RT-PCR） in 34 cases of NSCLC and 14 cases of non-neoplasia tissue. Results The expression rate of FFEN in normal lung tissues was 92. 8% （13/14） which was significantly higher than that in tissues of non-smaU cell lung cancer 35% （12/32） （P 〈0.05 ）. The expression of PTEN gene was associated with pathological styles and pathological grade in lung cancer patients （P 〈0.001）. Conclusion There is a higher incidence of negative expression of PTEN mRNA in NSCLC,which indicates that PIEN gene inactivation may play an important role in the genesis and development of NSCLC.