羌活和独活药材气相色谱指纹图谱的建立及鉴别

Establishment of chromatographic fingerprint and identification of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis by GC

目的为羌活和独活药材的鉴别提供科学依据。方法采用气相色谱(GC)-氢火焰离子化检测法。色谱柱:DB-1毛细管柱(30 m×0.25 mm×0.25μm);程序升温:起始柱温50℃,8℃.min-1升至85℃保持12 min,4℃.min-1升至155℃保持18 min,12℃.min-1升至250℃保持5 min;气化室温度:250℃;载气:氮气;流速:1.2 mL.min-1;进样量:1.0μL;分流比:10∶1;检测器温度:270℃。结果建立了羌活和独活药材的GC指纹图谱;对不同产地药材分别进行了相似度计算;指纹图谱和相似度计算结果显示,羌活和独活药材挥发油含量具有明显区别。结论本方法可用于羌活和独活药材的定性鉴别。

Objective To provide evidence for identification of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis. Methods The gas chromatography （GC） system consisted of DB-1 column （ 30 m× 0.25 mm × 0.25μm） and hydrogen flame ionization detector（FID）. The temperature of column was 50 ℃ after injection then programmed at 8 ℃·min^-1 to 85 ℃ for 12 min and at 4 ℃·min^-1 to 155℃ for 18 rain and at 12 ℃ .min^-1 to 250 ℃ for 5 min. The temperatures of gasification and FID were 250 ℃ and 270 ℃. The carrier gas was N2 at a flow rate of 1.2 mL·min^-1. The inlet volume was 1.0μL. The split ratio was 10： 1. Results The GC fingerprints of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were established and the similarities were respectively calculated. Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis showed evident difference based on fingerprints and similarities. Conclusion The method can effectively identify Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis.