RP-HPLC法同时测定茵陈中5种化学成分的含量

Simultaneous determination of contents of five compounds in Herba Artemisiae Capillariae by RP-HPLC

目的建立同时测定茵陈中芦丁、金丝桃苷、异槲皮苷、4，5-O-二咖啡酰奎宁酸、异鼠李素-3-O-葡萄糖苷含量的方法，为茵陈药材的质量控制提供依据。方法采用RP-HPLC法。色谱柱：Luna C18柱（250mm×4．6mm，5μm）；流动相：乙腈-体积分数0.04％磷酸水溶液（体积比17：83）；检测波长：345nm。结果芦丁、金丝桃苷、异槲皮苷、4，5-O-二咖啡酰奎宁酸、异鼠李素弓一O-葡萄糖苷质量浓度分别在0．942～18．84mg·L^-1（r=0．9995）、1．190～23．79mg·L^-1（r=0．9995）、1．107～22．14mg·L^-1。（r=0．9994）、56．78～1．136×10^3mg·L^-1。（r=0．9990）、0．6219～12．44mg·L^-1（r=0．9998）内与峰面积呈良好的线性关系（n=5）；方法回收率（n=9）分别为98．1％、100．7％、98．4％、100．2％、101．7％。结论该方法可用于茵陈药材的质量控制。

Objective To establish a method for simultaneous determination of rutin, hyperin, isoquercitrin, 4,5-O-dicaffeoyl quinic acid and isorhamnetin-3-O-glucoside in Herba Artemisiae Capillariae. Methods A RP-HPLC method detected by UV wavelengths of 345 nm were developed. The system consisted of a C18 column（250 mm×4.6mm,5 μn） taking a mixture of acetonitrile and 0.04% phosphoric acid as the mobile phase. Results The linear response range was 0. 942 0 - 18.84mg·L^-1（ r = 0. 999 5 ）, 1. 190 - 23.79 mg·L^-1（r=0.9995）,1.107-22.14mg·L^-1（r=0.9994）,56.78-1.136×10^3mg·L^-1（r= 0. 999 0） and 0.621 9 - 12.44mg·L^-1（ r = 0. 999 8 ）, respectively （ n = 5）. The average recoveries （ n = 9） of rutin, hyperin, isoquercitrin, 4,5-O-dicaffeoyl quinic acid and isorhamnetin-3-O-glucoside were 98.1%, 100.7% ,98.4%, I00.2% and 101.7%, respectively. Conclusions The assay demonstrates that the method has adequate accuracy and selectivity to measure the five chemical constituents in Herba Artemisiae Capillariae.