昆虫取食和药剂熏蒸对马尾松针叶脱落酸和茉莉酸含量的影响

Effects of Dendrolimus punctatus feeding and methyl jasmonate(MeJA)-or terpenes fumigation on abscisic acid and jasmonic acid contents in Pinus massoniana seedling needles

脱落酸(ABA)和茉莉酸(JA)在诱导植物产生抗逆性过程中具有重要作用.以马尾松为试验材料,用GC/MS检测了马尾松毛虫咬食、外源茉莉酸甲酯(MeJA)熏蒸和萜烯混合物熏蒸处理4 h后,马尾松损伤针叶、相邻姊妹针叶(处理同一轮未受损伤枝条)、系统上枝针叶(处理枝上一轮枝条)和系统下枝针叶(处理枝下一轮枝条)中ABA和JA含量的变化.结果表明,虫害4 h后,马尾松损伤针叶、相邻姊妹针叶、系统上枝针叶和系统下枝针叶的ABA和JA含量均明显升高.用10μmol.L-1MeJA熏蒸和10μmol.L-1萜烯混合物熏蒸健康植株后,各部位枝条针叶中ABA和JA含量明显升高.说明ABA和JA是马尾松苗木伤反应信号传导途径中的重要信号分子,参与了马尾松苗木系统抗性的形成过程.

Abscisic acid （ABA） and Jasmonic acid （JA） play an important role in inducing the stress-resistance of plants. In this study, parts of the needles on a ring of Pinus massoniana seedling shoots were subjected to 4 hours Dendrolimus punctatus feeding or 4 hours fumigation with 10umol·L^-1 of methyl jasmonate （MeJA） or terpenes, and the ABA and JA contents in treated needles, untreated neighboring sister needles, and untreated needles above and below the ring were determined by GC/MS. An obvious increase of ABA and JA contents was observed in all of the needles, whether they were treated or not, illustrating that ABA and JA were the vital signaling molecules in the wound signal transduction pathway, and participated in the formation of systematic resistance of P. massoniana seedlings.