姜黄素对SiO_2诱导小鼠矽肺模型TNF-α、TGF-β1表达的影响

Effects of curcumin on TNF-α and TGF-β1 in serum and lung tissue of SiO_2-induced fibrosis in mice

目的:研究姜黄素对SiO2所致小鼠矽肺模型血清及肺组织中肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)表达的影响。方法:将实验小鼠随机分为5组,即假手术组、模型组、姜黄素干预组(高、中、低剂量)。于姜黄素干预第14天、42天后分别每组各处死6、9只,采用ELISA法测定肺组织和血清中TNF-α、TGF-β1的含量。结果:模型组的肺组织炎症反应、纤维化程度较明显;与假手术组比较,肺组织匀浆、血清中TGF-β1、TNF-α含量均明显升高(P<0.01)。给予姜黄素干预后,可以不同程度地降低肺组织匀浆及血清中TGF-β1、TNF-α的含量(P<0.05),同时纤维化程度减轻。结论:姜黄素能降低SiO2致小鼠矽肺模型肺组织和血清中TNF-α、TGF-β1水平,可能通过下调上述细胞因子水平而发挥抗肺纤维化作用。

AIM： To study the effects of curcumin on TNF-α and TGF-β1 in serum and lung tissue of SiO2-induced fibrosis in mice. METHODS： 75 mice were divided into five groups. After treated with curcumin 6 and 9 mice of each group were sacrificed on day 14 and 42 respectively to take their blood and lung tissue. The level of TNF-α and TGF-β1 was observed by ELISA. RESULTS： The infection reaction of lung tissue and fibrosis in model group was obvious. Compared with sham operation group, TNF-α and TGF-β1 in serum and lung tissue increased significantly（ P 〈 0.01）, but decreased in different degrees after treated with curcumin （ P 〈 0.05 ）. CONCLUSION： Curcumin can decrease the level of TNF-α and TGF-β1 in serum and lung tissue of SiO2-induced fibrosis in mice and have the anti-fibrosis role by deregulating cytokine level.